人参高产发根无性系的筛选及其高效液体培养  

Selection and High efficient Culture of High yielding Hair Root Clone of Ginseng

作　　者：赵寿经[1] 杨振堂[1] 李昌禹[1] 臧埔 赵家平[1] 

机构地区：[1]中国农业科学院特产研究所,吉林132109

出　　处：《中国农业科学》2000年第5期103-105,共3页Scientia Agricultura Sinica

基　　金：中国农业科学院国际合作基金资助项目

摘　　要：In the co culture of root explants of ginseng and A 4 strain of Agrobacterium rhizogenes,the hairy root of ginseng transformed by A 4 strain were induced.By means of determination for growth rate,the high yielding hair root clone R98111 was selected.It achieved the most growth rate on a liquid 1/2 MS medium (25℃,in a shaker at 110r/min )and its growth rate increased by 31.2 fold in 28 days.Agropine and mannopine were detected in the R98111 clone by high voltage paper electrophoresis.In the liquid fermented culture of 2.0 litre medium,the yield of the hairy roots R98111 clone amounted to 38.29g(dry weight)in 28 days.In the co culture of root explants of ginseng and A 4 strain of Agrobacterium rhizogenes,the hairy root of ginseng transformed by A 4 strain were induced.By means of determination for growth rate,the high yielding hair root clone R98111 was selected.It achieved the most growth rate on a liquid 1/2 MS medium (25℃,in a shaker at 110r/min )and its growth rate increased by 31.2 fold in 28 days.Agropine and mannopine were detected in the R98111 clone by high voltage paper electrophoresis.In the liquid fermented culture of 2.0 litre medium,the yield of the hairy roots R98111 clone amounted to 38.29g(dry weight)in 28 days.

关 键 词：人参 RI质粒 转化 无性系 高效液体培养 

分 类 号：S567.51[农业科学—中草药栽培]