玉米大斑病菌HT-毒素对玉米细胞膜透性的影响  

The effect of HT-toxin produced by Exserohilum turcicum on membrane leakage of corn

作　　者：董金皋[1] 韩建民[1] 闫淑娟[1] 

机构地区：[1]河北农业大学植物病理系,河北保定071001

出　　处：《河北农业大学学报》2000年第3期59-64,共6页Journal of Hebei Agricultural University

基　　金：河北省自然科学基金 !(397132 ;3980 98);河北农业大学 9816科技重点课题

摘　　要：玉米大斑病菌HT -毒素粗毒素、通过硅胶G柱层析获得的组分I、5 -羟甲基 - 2 -呋喃甲醛对玉米细胞膜都具有明显的损伤作用 ,且与毒素浓度和处理时间呈正相关。试验发现 ,来自 2号小种的HT -粗毒素因含有组分IV ,对OH43Ht1玉米细胞膜的损伤要比组分I和 5 -羟甲基 - 2 -呋喃甲醛强的多。HT -毒素中外源加入强氧化剂H2 O2后 ,可提高毒素对细胞膜的破坏作用 ,尤其是 5 -羟甲基 - 2 -呋喃甲醛加入H2 O2 后对叶片细胞膜的破坏作用更加明显。结论初步判断 ,HTHT toxin could strongly destroy the membrane permeability and induce leakage of conductivity of corn leaf cells. There was a high correlation between membrane permeability and toxin conc. & toxin treated time. H 2O 2, a powerful oxidative agent, could increase the detriment efficiency of HT-toxin to membrane of corn leaf cells after it was added to HT-toxin, and it was more distinct in OH43Ht1 corn when H 2O 2 was added to standard HT-toxin. It was concluded that cell membrane of corn might be primary action site of HT-toxin.

关 键 词：玉米大斑病 HT-毒素 细胞膜透性 

分 类 号：S435.131[农业科学—农业昆虫与害虫防治]