植物提取物中茉莉酸定性定量分析  

Identification and Quantitative Determination of Jasmonic Acid in Plant Extracts

作　　者：金尉[1] 余雯静[1] 郑利[1] 

机构地区：[1]苏州出入境检验检疫局,江苏苏州215104

出　　处：《香料香精化妆品》2013年第6期10-13,共4页Flavour Fragrance Cosmetics

摘　　要：建立了植物提取物中茉莉酸的高效液相色谱（HPLC）-三重四极杆复合线性离子阱（QTrap）质谱测定方法，样品经提取后，采用反相色谱柱进行保留、分离，大气压电离源将样品离子化，经串联四极杆复合线性离子阱质谱进行定性和定量检测。茉和酸在0.1-100ng/mL浓度范刚内呈良好线性．实际样品的最低榆出限为0．05ng／mL，最低定量限为0.1ng/ML.通过标准添加的方式，考察高、中、低三个水平的样品凹收率以及相对标准偏差（n=15），得到三个水平的平均回收率分别为80．57％、95．24％、98．48％，相对标准偏差为1．59％、1．26％、1．04％。A method for determination of jasmonic acid in plant extracts has heen developed. The plant sample was extracted and separated using reversed phase silica columns, then determined by LC-MS/MS. The calibration curves of jasmonie acid showed good linearity in the range of 0.1 to 200 ng/mL, the detection limit of method was 0.05 ng/mL, and the limit of quantifieation was 0. I ng/mL. After adding standard solution to sample, the average recoveries and RSDs of high, medium, low level （n = 15） were investigated. The average recoveries of three levels were 80. 57%, 95.24% and 98.48%, respectively, and the RSDs were 1.59%, 1.26% and 1.04% , respectively.

关 键 词：茉莉酸 植物提取物 HPLC—QTrap 

分 类 号：TQ654.2[化学工程—精细化工]