草果精油萃取方法及清除DPPH自由基能力研究  

Extraction Method and DPPH Scavenging Capacity of Essential Oil from Amomum tsao-ko

作　　者：孟大威[1] 李伟[1] 王鹏君[2] 重松正己 任慧峰[4] 陆占国[1] 

机构地区：[1]哈尔滨商业大学食品工程学院/黑龙江省高校食品科学与工程重点实验室,黑龙江哈尔滨150076 [2]燕山大学环境与化学工程学院,河北秦皇岛066004 [3]日本萜化学(株) [4]东京海洋大学海洋环境学科

出　　处：《香料香精化妆品》2013年第6期17-22,共6页Flavour Fragrance Cosmetics

摘　　要：采用水蒸气蒸馏-乙醚萃取法和Clevenger法萃取草果挥发性成分,分别以1.12%和1.87%的平均得率获得了精油。采用GC/MS分析方法从水蒸气蒸馏-乙醚萃取获得的精油中检测出30种成分,解析鉴定出其中29个成分,总相对含量占精油全部组分的99.619%,主要成分为1,8-桉树脑(41.433%)、α-水芹烯(7.882%)、2-异丙基苯甲醛(5.930%)。Clevenger法所得精油检测出38种成分,解析鉴定出其中32个成分,总相对含量占精油全部组分的98.395%,主要成分为1,8-桉树脑(40.891%)、α-水芹烯(9.769%)、2-异丙基苯甲醛(6.988%)。两种方法获得的精油都具有明显的清除DPPH自由基能力,在浓度为10 mg/mL时,以水蒸气蒸馏-乙醚萃取法和Clevenger法所获得的精油对DPPH的清除率分别为80.0%和75.8%。The essential oils were obtained in yields of 1. 12% and 1.87% from Amomum tsao ko by steam distillation - dieth yl ether extraction and Clevenger method respectively. The chemical components of two essential oils were analyzed by GC/ MS. When the essential oil obtained by steam distillation - diethyI ether extraction method was analyzed, 30 components were detected and in which 29 components that occupied 99. 619% （relative content, and so on） of the total constituents were（dentifled, the main components were 1 , 8 eineol （ 41. 433% ）, a-phellandrene （ 7. 882% ） arid 2 isopropylbenzaldehyde （ 5. 930% ）. For the essential oil obtained by Clevenger method, a total of 38 components were detected, in whieh 32 components that account for 98. 395% were identified. The main components were 1,8-cineole （41）. 891%） （mass fraction）, ce phellandrene （9.769%） and 2-isopropylbenzaidehyde （6.988%）. The ability of scavenging DPPH of both essential oils were studied, and the result showed that they had considerable effect of scavenging DPPH, the scavenging rate were 80. 0% and 75.8%, respec- tively, when 10. 0 mg/mL of oils were used.

关 键 词：草果 水蒸气蒸馏萃取法Clevenger法 精油 气相色谱 质谱法DPPH 自由基 

分 类 号：TQ654.2[化学工程—精细化工]