星点设计-响应面法优化北五味子多糖提取工艺  

Optimal extraction of polysaccharide from schisandra chinensis by central composite design and response surface methodology

作　　者：金兰[1] 张海晶[1] 陈大忠[1] 

机构地区：[1]黑龙江中医药大学中医药研究院,哈尔滨150040

出　　处：《哈尔滨商业大学学报（自然科学版）》2013年第6期649-652,666,共5页Journal of Harbin University of Commerce：Natural Sciences Edition

摘　　要：利用星点设计-响应面法对北五味子多糖提取工艺进行优化.以提取温度、提取时间、溶剂倍数为自变量,多糖得率为因变量,通过对自变量各水平的多元线性回归及二项式拟合,用响应面分析法选取最佳工艺,并进行预测分析.确定最优工艺条件为:提取温度为92℃,提取时间为171.6 min,溶剂倍数为21.55倍,提取3次;多糖得率提取预测值与实际值偏差为-0.87%.星点设计-响应面法优选五味子多糖提取工艺方法简单合理,稳定,精密度高,预测性好,为北五味子多糖的生产工艺提供依据.A response surface methodology was established to optimize the extracting technol- ogy of polysaccharides from schisandra chinensis. The independent variables were extraction temperature, refluxing time and solvent multiple, and the dependent variable was extraction rate of total polysaccharides in S. chinensis. Linear or non-linear mathematic models were used to estimate the relationship between independent and dependent variables. Central com- posite design and response surface methodology was carried out by comparing the observed and predicted values. The optimum conditions of extraction process were as follows. Extrac- tion temperature was 92 ~C, refluxing time was 171.2 rain, solvent multiple （liquid to solid ratio） was 21.55： 1, and 3 times for extraction. Bias between observed values and predicted values was 0.8%. The optimum extraction technology is simple, reliable, stable and highly predictive. This method can be used as a reference for the extraction of S. chinensis polysac- charide.

关 键 词：北五味子 多糖 星点设计 响应面法 提取工艺 

分 类 号：R283.6[医药卫生—中药学]