HBV慢性感染者血清中MBL、MASP和C3b水平检测的临床意义  

Study of the expression levels of MBL,MASP,and C3b and the clinical significance for hepatitis B infection patients

作　　者：许大成[1] 古丽米娜.玉山 张朝霞[1] 严雪梅[1] 蒋静[1] 

机构地区：[1]新疆医科大学第一附属医院检验科,新疆乌鲁木齐830054 [2]新疆医科大学第一附属医院输血科,新疆乌鲁木齐830054

出　　处：《国际检验医学杂志》2013年第24期3305-3307,共3页International Journal of Laboratory Medicine

基　　金：国家自然科学基金资助项目(30660718);新疆维吾尔自治区病原生物学重点学科资助项目(BYX11001)

摘　　要：目的研究MBL、MASP和C3b在不同感染程度的乙肝患者中的表达及其临床意义。方法乙肝患者共140例,依据血清中HBeAg和ALT的水平分为6组:A组(HBeAg阳性,ALT正常)、B组(HBeAg阴性,ALT正常)、C组(HBeAg阳性,ALT轻度/中度升高)、D组(HBeAg阳性,ALT高度/重度升高)、E组(HBeAg阴性,ALT轻度/中度升高)、F组(HBeAg阴性,ALT高度/重度升高)。采用ELISA法检测健康对照(G组)及患者血清中MBL、MASP和C3b的水平,并对这三者之间的水平做相关性分析。结果 A组的MBL水平显著高于C组和D组(P<0.05),G组的MBL水平高于除B组外的其余5组(P<0.05)。F组的MASP水平显著高于A、C、D组,同时其显著低于B、G组(P<0.05),D组的MASP水平显著低于其余6组(P<0.05),G组的MASP水平显著高于其余6组(P<0.05)。D组和F组的C3b水平显著高于A、B、C、E组(P<0.05),但D、F组之间并无显著差异(P>0.05),G组的C3b水平显著低于其余6组(P<0.05),MBL与MASP之间存在中度正相关性,而C3b与MASP、MBL之间存在弱的负相关性(P<0.05)。结论 MBL与机体清除HBV有关,高水平C3b可能会提高乙肝病变程度,MASP水平异常降低与乙肝患者肝损伤程度加重、病毒感染和复制水平加剧均有关系。Objective To study the expression of MBL, MASP and C3b in patients with hepatitis B virus infection with different degree and its clinical significance. Methods 140 patients with chronic hepatitis B were divided into six groups based on HBeAg and ALT levels ： group A （ HBeAg positive, ALT normal）, group B （ HBeAg negative, ALT normal）, group C （ HBeAg positive, ALT mild/moderate rise）,D group（HBeAg positive, ALT height/severe rise）, group E（HBeAg negative, ALT mild/moderate rise）, group F（HBeAg negative, ALT height/severe increase）. ELISA test was used to detect the expression levels of MBL, MASP and C3b in normal control（namely group G） and patient groups,and the correlations with MBL,MASP and C3b were analyzed. Results MBL level of group A was higher than those of group C and D（P〈0.05）,and which of control group G was higher than the other five groups except group B（P〈0.05）. MASP level in group D was the lowest while group G was the highest,which in group F was higher than those in group A and C（P〈0.05）. C3b level in control group G was the lowest（P〈0.05） ,which in group D and F showed no statistical difference（P〉0.05）, but both of them were higher than other 4 patient＇s groups. MBL and MASP had moderate positive correlation,C3b showed weakly negative correlation with MASP and MBL. Conclusion MBL removes HBV,its low level expression will increase the severity of hepatitis B. The high level of C3b may worsen HBV infection. Hepatitis B patients MASP level may have relation with the level of patients abnormal reduce and liver damage degree,virus infection and replication.

关 键 词：甘露糖结合凝集素 补体C3b 甘露糖结合凝集素相关丝氨酸蛋白酶 肝炎病毒 乙型 

分 类 号：R512.62[医药卫生—内科学]