免疫性血小板减少性紫癜患者单个核细胞中microRNA-30a表达增高及其意义  

Upregulation of microRNA-30a in peripheral blood mononuclear cells from patients with immune thrombocytopenic purpura and its clinical significance

作　　者：叶辛[1] 张蕾[2] 石磊[2] 谷明莉[2] 张薇薇[2] 张建荣[2] 秦琴[2] 钱宝华[1] 邓安梅[2] 

机构地区：[1]第二军医大学长海医院输血科,上海200433 [2]第二军医大学长海医院实验诊断科,上海200433

出　　处：《国际检验医学杂志》2013年第24期3308-3309,3311,共3页International Journal of Laboratory Medicine

基　　金：国家重点基础研究发展计划(973)资助项目(2013CB531606);国家自然科学基金资助项目(81273282;81202353;30972730);上海科委基金资助项目(11JC1410902);吴阶平基金资助项目(320.6750.13147);上海杨浦区人才发展资金资助项目(鼎元资金);长海医院基金资助项目(CH125530300)

摘　　要：目的探讨microRNA-30a是否参与了免疫性血小板减少性紫癜(ITP)的发病机制。方法采用实时荧光定量逆转录-聚合酶链反应(FQ-RT-PCR)技术检测38例ITP患者及35例健康者外周血单个核细胞(PBMC)中microRNA-30a和Lyn mRNA的相对表达量,并进行统计分析。结果 ITP患者外周血PBMC中microRNA-30a表达较健康对照组明显增高(P<0.01),且与血小板计数呈负相关(r2=0.42,P<0.05);ITP患者外周血PBMC中Lyn mRNA表达较健康对照组明显降低(P<0.01),且与microRNA-30a呈负相关(r2=0.33,P<0.01)。结论 microRNA-30a可能通过Lyn参与了ITP的发病机制。Objective To investigate whether microRNA-30a may play a role in the pathogenesis of immune thrombocytopenic purpura（ITP）. Methods The expression of microRNA-30a in the peripheral blood mononuelear cells（PBMCs） in 38 ITP patients and 35 healthy individuals were detected by RT-PCR. The confirmed target expression of microRNA-30a and Lyn mRNA were de- tected. The association between microRNA-30a and platelet count of ITP was analyzed. Results The expression of microRNA-30a in PBMCs from patients with ITP was increased significantly in contrast to the healthy controls（P〈0. 01 ）, and was negatively correlated with platelet count. The expression of Lyn mRNA was significantly reduced, compared to control subjects（P〈0.01） ,and was negatively associ- ated with microRNA-30a. Conclusion MicroRNA-30a may play a role in the pathogenesis of ITP and was a potential marker for it.

关 键 词：免疫性血小板减少性紫癜 microRNA-30a 实时荧光定量逆转录PCR 

分 类 号：R554.8[医药卫生—血液循环系统疾病]