抗中性粒细胞胞浆抗体检测在肺纤维化疾病中应用研究  

Study on the detection of antineutrophil cytoplasmic antibodies in pulmonary fibrosis

作　　者：赵春[1] 符布清[1] 杨丽[1] 陈秋霞[1] 

机构地区：[1]江苏省中医院检验科,江苏南京210029

出　　处：《国际检验医学杂志》2013年第24期3332-3334,共3页International Journal of Laboratory Medicine

摘　　要：目的探讨肺纤维化疾病患者的抗中性粒细胞胞浆抗体(ANCA)与血清胱抑素C(CysC)和高敏C反应蛋白等指标之间的变化规律。方法检测69例肺间质纤维化伴感染患者和30例健康对照组的ANCA及血尿素氮、肌酐、CysC和高敏C反应蛋白等指标。ANCA检测先应用间接免疫荧光法,并用免疫印迹法确定靶抗原。生化指标用全自动生化分析仪测定。白细胞和血小板用血细胞分析仪测定。结果肺纤ANCA(+)组CysC水平明显高于肺纤ANCA(-)组与健康对照组(P<0.05),而肺纤ANCA(-)组与健康对照组的CysC比较差异无统计学意义(P>0.05);肺纤ANCA(+)组与肺纤ANCA(-)组CRP水平明显高于与健康对照组(P<0.01),其他指标各组比较差异无统计学意义(P>0.05)。结论肺纤ANCA(+)可能伴有肾功能损伤,应注重肺纤ANCA(+)患者的肾功能监测。Objective To investigate variation between the anti neutrophil cytoplasmic antibody（ANCA） in patients with pulmo- nary fibrosis and serum cystatin C（CysC）and high sensitive C reactive protein index. Methods To determination the level of ANCA and serum urea nitrogen, creatinine,CysC and high sensitive C reactive protein and other indicators in patients with 95 cases of pul- monary interstitial fibrosis associated with infection and 30 cases of normal control group,indirect immunofluorescence was used to detect the ANCA（IIF） and Western blot,and biochemical indicators were determined by automatic biochemical analyzer. Results The levels of CysC of ANCA（＋） in patients with pulmonary fibrosis group were significantly higher than that of ANCA（-） pul- monary fibrosis and normal control group（P〈0.05）, and the levels of CysC of ANCA（-） in patients with pulmonary fibrosis group has no significant difference compared with the normal control group（P〉0.05） ;the levels of CRP in pulmonary fibrosis AN- CA（＋） group and pulmonary fibrosis ANCA（- ） group were significantly higher than that of in normal control group（P〈0.01）, the other indexes of all groups had no significant difference（P〉0.05）. Conclusion Pulmonary fibrosis ANCA（＋） may be associ- ated with renal function damage,we should pay attention to the determination of renal function in pulmonary fibrosis ANCA（＋） patients.

关 键 词：肺纤维化 抗体 抗中性白细胞胞质 半胱氨酸蛋白酶抑制剂 C反应蛋白质 

分 类 号：R446.6[医药卫生—诊断学]