基于层层自组装技术的纳米电化学传感界面构建及其免疫应用  

Construction of Biomimetic Interface by Layer-by-Layer Self-Assembly Technique for Electrochemical Amperometric Immunosensing Applications

作　　者：张立[1] 范丽霞[1] 梁汝萍[1] 邱建丁[1] 

机构地区：[1]南昌大学化学系,南昌330031

出　　处：《分析化学》2013年第12期1795-1800,共6页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金(21105044;21265012);教育部新世纪优秀人才支持计划(NCET-11-1002)项目资助

摘　　要：将壳聚糖-二茂铁/多壁碳纳米管复合物（CS-Fc/MWNTs）与金纳米粒子（AuNPs）通过层层自组装（LBL）技术固定于3-巯基丙烷磺酸钠盐（MPS）修饰电极表面,形成的三维多层网状结构增加了电极表面的活性位点,可高效吸附抗体,从而制得免标记的电化学免疫传感界面。以乙肝表面抗原（HBsAg）为例对传感器的性能进行考察,结果表明,传感器对HBsAg检测的线性范围为0.1～350μg/L,检出限为0.037μg/L。免疫传感器对不同浓度HBsAg的测定结果与ELISA法测得数据的相对偏差在-4.5%～8.3%之间,表明此免疫感器对HBsAg测定的可靠性高,适用于临床样品的检测。A novel nontoxic biomimetic interface for developing label-free amperometric immunosensor based on layer-by-layer self-assembly of positively charged ferrocene-branched chitosa_n/multiwalled carbon nanotubes （CS-Fc/MWNTs） and negatively charged gold nanoparticles （Au NPs ） onto 3-mercaptopropane-sulfonic, sodium salt （MPS）-modified gold electrode surface was described. The constructed three-dimensional mutilayer network structure provides more stereo sites and ideal platform for further immobilization of antibody to prepare immunosensors. Using hepatitis B surface antigen （HBsAg） as a model, the proplsed immunosensor exhibits a specific response to HBsAg in the concentration range of 0.1-350 μg/L with a detection limit of 0.037 μg/L （S/N=3）. The relative error between the results obtained from the immunosensor and the standard method proposed by ELISA was --4.5%-8.3%, indicating that the two methods were in acceptable agreement and the proposed method could be applied to the clinical determination of HBsAg in human serum for clinical diagnosis.

关 键 词：安培免疫传感器 层层自组装技术 壳聚糖-二茂铁 多壁碳纳米管 金纳米粒子 

分 类 号：TP212[自动化与计算机技术—检测技术与自动化装置]