十二烷基硫酸钠和硼酸协同增敏铽荧光探针及其测定沙拉沙星  

Synergistic Fluorescence Enhancement of Terbium Probe with Sodium Dodecyl Sulfate and Boric Acid and Its Determination of Sarafloxacin

作　　者：于永丽[1] 张敏[1] 张永芳[1] 徐淑坤[1] 

机构地区：[1]东北大学理学院,沈阳110819

出　　处：《分析化学》2013年第12期1951-1952,共2页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金(No.20875011)资助项目

摘　　要：1引言 沙拉沙星（SARA）是继恩诺沙星之后又一种新的动物专用氟喹诺酮类药物，是防治畜禽疾病的理想药物。目前，测定SARA的方法主要有高效液相色谱法、荧光分析法和毛细管电泳串联质谱法等。A spectrofluorimetric method for the determination of Sarafloxacin （SARA） based on the fluorescence enhancement of Tb3＋-SARA complex in the presence of sodium dodecyl sulfate （SDS） and boric acid was developed. The effect of various experimental parameters, such as pH value, the type and amount of surfactant, the type and amount of buffer solution and the concentration of Tb3＋, on the fluorescence intensity of the complex was investigated. Under the optimum conditions, the enhanced fluorescence intensity of the system showed a good linear relationship with the concentration of SARA in the range of 0. 100-1.80 × 103 μg/L with a correlation coefficient of 0.9994. The detection limit of SARA was found to be O. 02 μg/L, and relative standard deviation （RSD） was 1.8% （c= 10.0 μg/L, n= 11 ）. The method has been successfully applied to the determination of SARA in milk samples with the recoveries of 95.5%-101%.

关 键 词：沙拉沙星 十二烷基硫酸钠 荧光探针 协同增敏 测定 氟喹诺酮类药物 高效液相色谱法 硼酸 

分 类 号：S859.84[农业科学—临床兽医学]