电化学DNA芯片快速检测临床病原微生物的研究进展  

Research Development of Electrochemical DNA Chip Rapid Detection of Clinical Pathogenic Micro-organisms

作　　者：李世红[1] 胡大春[2] 

机构地区：[1]昆明医科大学,昆明650504 [2]昆明市第一人民医院检验科,昆明650100

出　　处：《医学综述》2013年第24期4508-4511,共4页Medical Recapitulate

摘　　要：电化学DNA芯片技术是集合聚合酶链反应、传感技术及生物芯片三大技术优点来检测目标核酸序列的新方法。目前,因电化学DNA芯片操作简单快速、特异性好、敏感性高、检测费用低、可同时检测多个样本、易于微型化和自动化等优点被用于多个领域,如传染病快速检验、疾病基因诊断、环境监测、食品安全、流行病学研究、法医鉴定及临床病源微生物的检测诊断等。该文对电化学DNA芯片工作原理及其在临床病源微生物检测诊断中的应用研究进展进行综述。Electrochemical DNA chip technology is a new method with integrated advantages of PCR, sensor and biological chip to detect target nucleic acid sequence. At present,the electrochemical DNA chip has been used in many fields for its simple and fast operation,specificity,sensitivity,low detection cost, simultaneously detection of multiple samples,easy miniaturization and automation,such as the rapid testing of infectious diseases,genetic diagnosis,environmental monitoring,food safety,epidemiological studies,forensic identification and detection diagnosis of clinical pathogenic microorganism. Here is to make a review of the progress of the operating principle of electrochemical DNA chip and its application in diagnosis of the clinical pathogenic micro-organisms.

关 键 词：微生物 电化学传感器 DNA探针 电化学DNA芯片 

分 类 号：Q812[生物学—生物工程]