SIV、PCV-2、PRRSV三重PCR检测方法的建立  

Establishment and Application of a triplex PCR for Detection of SIV,PCV-2 and PRRSV

作　　者：陈胜锋[1] 徐振娜[2] 王丙云[1] 计慧琴[1] 陈志胜[1] 

机构地区：[1]佛山科学技术学院动物医学系,广东佛山528231 [2]东莞市动物疫病预防控制中心,广东东莞523107

出　　处：《动物医学进展》2013年第12期42-45,共4页Progress In Veterinary Medicine

基　　金：佛山市科技专项发展基金项目(2009030);广东省佛山市禅城区科技开发专项资金项目(2011A1063)

摘　　要：为建立猪流感病毒（SIV）、猪圆环病毒2型（PCV-2）、猪繁殖与呼吸综合征病毒（PRRSV）单一或混合感染的快速检测方法,针对病毒保守区设计3对特异性引物用于扩增SIV、PCV-2与PRRSV基因的相应片段,并通过优化反应条件,建立了SIV、PCV-2、PRRSV三重PCR检测方法,其最低检测限度为10-4-10-5数量级。用三重PCR和单项PCR/RT-PCR检测40份临床病料,符合率为100%。提示建立的三重PCR检测方法能够对SIV、PCV-2、PRRSV单一或混合感染的临床样品进行快速鉴别检测,具有一定的临床应用价值。According to the sequences reported in GenBank of virus conserved sequences,three pairs of primer were synthesized to amplify target sequences of SIV、PCV-2、PRRSV.Through the optimization of reaction conditions and the detection of clinical cases,the SIV,PCV-2and PRRSV triplex PCR was established.The results show that the triplex PCR had the minimum detection limit10-4-10-5.40clinical samples were detected by triplex PCR and single PCR/RT-PCR,the consistent rate was 100%.The results suggest that：The triplex PCR can be used for the detection of clinical samples with single or coinfections with three kinds of viruses.

关 键 词：猪流感病毒 猪圆环病毒2型 猪繁殖与呼吸综合征病毒 三重PCR 

分 类 号：S852.65[农业科学—基础兽医学]