抗PRRSV和PCV-2-Cap蛋白特异性IgY抗体对保育猪生长和免疫的影响  

Effects of Specific IgY Antibodies Against PRRSV and PCV-2-Cap Protein on Growth Performance and Immunity of Weaning Piglets

作　　者：鲍伟华 周雪荣 卢黎明 孙鹏烈 樊莉 方维焕[4] 李肖梁[4] 

机构地区：[1]宁波市畜牧兽医局,浙江宁波315000 [2]浙江欣欣饲料股份有限公司,浙江嘉兴314000 [3]宁波市鄞州区畜牧兽医站,浙江宁波315040 [4]浙江大学动物预防医学研究所,浙江省动物预防医学重点实验室,浙江杭州310058

出　　处：《动物医学进展》2013年第12期74-79,共6页Progress In Veterinary Medicine

基　　金：浙江省重大科技专项重点农业项目(2012C12003-2);杭州市科技局农村农业科研推广专项(20110232B22);宁波市农业科技攻关项目(2011C1003)

摘　　要：应用特异性抗猪繁殖与呼吸综合征病毒（PRRSV）和猪圆环病毒2型（PCV-2）Cap蛋白卵黄免疫球蛋白（IgY）饲喂保育猪,观察其对保育猪生长和免疫性能的影响。经抗体检测表明,53日龄时,PRRSV和PCV-2抗体阴性率0.5%,试验组比对照组分别高15%和5%。在53日龄和60日龄时,保育猪PRRSV阳性抗体S/P均值,试验组比对照组分别低15.59%和24.53%。保育猪46日龄-60日龄之间,阳性PCV-2抗体S/P均值试验组比对照组分别低15.42%、19.66%和12.70%。保育猪转栏时,试验组比对照组平均末重高12.74%（P〈0.01）,平均日增重提高16.90%（P〈0.01）,成活率高1.67%。免疫组化结果显示,相对于空白组,添加组空肠和小肠组织中的PRRSV抗原的阳性表达几乎为0。试验结果为特异性抗体用于PRRSV和PCV-2的防控提供了参考。Weaning piglets were fed with specific egg yolk immunoglobulin（IgY）antibodies against porcine reproductive and respiratory syndrome virus（PRRSV）and porcine circovirus type 2（PCV-2）-Cap protein,of which on growth performance and immunity was investigated.The results indicated that PRRSV and PCV-2antibody negative rate was higher 15% and 5% in 0.5% groups than that of in blank groups at 53 days of age,respectively.Compared with blank groups,S/P average value of positive PRRSV antibody titer in 0.5% groups decreased 15.59% and 24.53% over 53 and 60 days of age,respectively.During 46to 60days of age,SP average value of postive PCV-2antibody titer of 0.5% groups were lower 15.42%,19.66% and 12.70% than that of in blank groups,respectively.Compared with blank groups,finial body weight,daily weight gain and survival rate of 0.5% groups were improved 12.74%（P0.01）,16.90%（P0.01）and 1.67% at weaning piglets,respectively.The expressions of PRRSV antigen in 0.5% groups were abolished in jejunum and small intestine with immunohistochemistry analysing,compared with the blank groups.The special IgY additive could provide a novel alternative approach to prevent and control diseases caused by PRRSV and PCV-2 infection in pigs.

关 键 词：猪繁殖与呼吸综合征病毒 猪园环病毒2型Cap蛋白 卵黄免疫球蛋白 保育猪 

分 类 号：S859.53[农业科学—临床兽医学]