雌激素膜受体GPR30在妊娠期关中奶山羊子宫中的分布  

Expression of Estrogen Membrane Receptor GPR30 in Uterus of Pregnant Guanzhong Dairy Goats

作　　者：覃初斌[1] 代盈盈[1] 王文丽[1] 刘红改[1] 赵慧英[1] 

机构地区：[1]西北农林科技大学动物医学学院,陕西杨凌712100

出　　处：《动物医学进展》2013年第12期124-127,共4页Progress In Veterinary Medicine

基　　金：抗病转基因牛新品种培育重点专项(2009ZX08007-008B)

摘　　要：为了探讨雌激素膜受体G蛋白偶联受体30(G protein-coupled receptor 30,GPR30)在妊娠期山羊子宫组织中的分布和表达变化规律,通过免疫组织化学SP法,分别对妊娠前期(1d^30d)、妊娠中期(31d^120d)和妊娠后期(121d^150d)关中奶山羊子宫中GPR30的分布及相对表达量的变化进行了研究。结果表明,GPR30免疫反应阳性产物在关中奶山羊子宫的子宫内膜、肌层和浆膜均有不同程度分布,主要位于细胞膜和细胞质,其中在子宫内膜腔上皮细胞和腺上皮中阳性细胞最多、着色最深,且随妊娠时期推移逐渐增强;肌层着色细胞染色较浅或无,浆膜层几乎无着色。子宫组织中GPR30相对表达量呈妊娠前期<妊娠中期<妊娠后期的趋势,妊娠早期表达量最低且极显著低于妊娠中期和后期(P<0.01),妊娠后期表达量达最高值,但与妊娠中期无显著差别(P>0.05)。GPR30在妊娠期关中奶山羊子宫的分布和表达具有一定的规律且主要通过对子宫内膜分泌的调节参与妊娠及分娩等生理活动。To investigate expression of G-protein coupled receptor 30 （GPR30）in uterus of goats during pregnancy. Immunochemistry SP method was used to detect the distribution of GPR30 and its protein a-bundance during the early pregnancy stage （1-30 d）, mid-term pregnancy stage （31-120 d） and late preg-nancy stage （121-150 d）. Different degree of GPR30 positive immunoreactions were observed in the endo-metrial layer, myometrium and serosa of uterus in dairy goats. GPR30 mainly located in the cell membrane and cytoplasm, especially in endometrial epithelial cells and glandular epithelium cells, while myometrium and serosa showed few or none immunoreactions. The staining was increased in endometrial epithelial cells and glandular epithelium with the progress of pregnancy. The relative level of GPR30 in uterus showed a trend of early pregnancy stage 〈 mid-term pregnancy stage 〈 late pregnancy stage, and the expression of GPR30 at the early stage of pregnancy was extremely lower than that of mid-term pregnancy stage and late pregnancy stage （P〈0.01）. GPR30 expression is maximal in the late pregnancy, but no significant differ-ence compared with mid-term pregnancy stage. GPR30 distribution and expression in the uterus of preg-nancy dairy goats showed certain regularity and mainly through the regulation of endometrial secretion in-volved in pregnancy and delivery and other physiological activities.

关 键 词：妊娠期 子宫 G蛋白偶联受体30 奶山羊 

分 类 号：S852.651[农业科学—基础兽医学] Q786[农业科学—兽医学]