富血小板血浆在促进兔烫伤创面愈合中bFGF VEGF的表达及意义  

Significance of platelet rich plasma in promoting bFGF and VEGF expression in would healing of rabbit buns

作　　者：胡以信[1] 袁杰 邹勇[1] 郭力[3] 刘兵[1] 

机构地区：[1]四川省宜宾市第二人民医院整形烧伤科,644000 [2]四川省宜宾市妇幼保健院儿科,644000 [3]泸州医学院附属医院整形烧伤科,四川泸州646000

出　　处：《重庆医学》2013年第35期4297-4298,4301,共3页Chongqing medicine

摘　　要：目的探讨富血小板血浆(PRP)在促进兔烫伤创面愈合碱性成纤维细胞生长因子(bFGF)、血管内皮生长因子(VEGF)的表达及意义。方法将24只大白兔分为对照组和实验组,对照组用磺胺嘧啶银(SD-Ag),实验组用PRP。在7、10、14d,两组分别随机选取4只大白兔麻醉处死后取材,行创面愈合率比较、HE染色及免疫组织化学检测。结果 7、10、14d实验组创面愈合率均较对照组高(P<0.05)。7d时,实验组肉芽组织灶显著,细胞及血管数量密集。10d时,实验组可见毛细血管量及成纤维细胞较对照组更为丰富。14d时,实验组显示大部分成纤维细胞转化为纤维细胞而毛细血管数减少。7、10dbFGF及VEGF阳性细胞数两组均增高,实验组明显高于对照组(P<0.05),而14dbFGF及VEGF阳性细胞数两组均逐步下降(P>0.05)。结论 PRP可以促进大白兔烫伤创面愈合,主要机制是明显增加早期创面中bFGF及VEGF表达。Objective To discuss the significance of platelet rich plasma in promoting bFGF and VEGF expression in would healing of rabbit buns.Methods The 24rabbits were randomly divided into control group and experimental group.The sulfadiazine silver paint to the wound for control group and the platelet rich plasma gel evenly spread to the wound for experimental group.At 7th,10th,14th day,4rabbits of each group were randomly selected to sacrificed after anesthesiaed,wound healing rate was compare in 2groups,HE staining and testimmunohistochemistry were conducted in each group.Results At 7th,10th,14th day,the wound healing rate of experimental group were higher than those of control group.At 7th d,the focal granulation,cells and vessels intensive were more significant in experimental group than in control group,at 10th day,the wound fibroblast cells and capillary number are more significant in experimental group,and at 14th day,the most of fibroblasts translate into fiber cell and the capillary number decreased,the fibroblasts proliferation was still active and fiber cell was less in the control group.At 7th,10th day,the expression of bFGF and VEGF were both higher,but experimental group is obvious higher than control group（P〈0.05）,but at 14th day,the expression of bFGF and VEGF were gradually declined and was not obvious statistics differences in the two group（P〈0.05）.Conclusion The platelet rich plasma could promote the big white rabbit scald wound healing,while the main mechanism maybe the expression of bFGF and VEGF increased in the early.

关 键 词：富血小板血浆 烫伤创面 碱性成纤维细胞生长因子2 血管内皮生长因子类 兔 

分 类 号：R644[医药卫生—外科学]