功能化超顺磁性Fe3O4纳米微粒的制备及在β-银环蛇毒素检测中的应用  

Preparation of Functionalized Superparamagnetic Fe3O4 Nanoparticles and its Application in Detection of β-bungarotoxin

作　　者：穆晞惠[1] 黄启斌[1] 童朝阳[1] 刘冰[1] 

机构地区：[1]防化研究院国民核生化灾害防护国家重点实验室,北京102205

出　　处：《纳米科技》2013年第6期72-77,共6页

摘　　要：采用微波共沉淀法制备超顺磁性Fe3O4纳米粒子并对其进行功能化修饰，探讨了制备功能化β—BGT磁性纳米微粒的最佳工作条件，建立了双抗体夹心磁分离酶免疫分析法检测β-BGT，结果表明，制备的功能化β—BGT磁性纳米微粒具有良好的活性，每mg磁微粒固定的生物素标记β—BGT多抗最大量为925μg。双抗体夹心磁分离酶免疫分析法检测β-BGT线性范围为0．062～125μg／L，线性回归方程为Y=0．705X＋1．017（R^2=0．991，n=12，P〈0．0001），检测限为O．062μg／L，具有较好的重现性。不同浓度的蓖麻毒素、相思子毒素、葡萄球菌肠毒素B对检测结果基本无干扰。Prepared superparamagnetic Fe3O4 nanoparticles by Microwave coprecipitation and functional modified the prepared nanoparticles. Discussed the optimum working conditions for preparing funetionalized β-BGT magnetic nanoparticles and established a double antibody sandwich magnetic affinity immunoassay for the detection of β-BGT. The results showed that the functionalized Fe3O4 magnetic nanoparticles had good activity,the maximum amount of fixed biotin-labeled anti-β-BGT PcAh per mg magnetic particles was 925μg. The linear relationship for the detection of β- BGT at a range of 0.062-125μg/L was obtained,fitting equation was Y=0.705X＋1.017（R^2=0.991 ,n=12,P〈0.0001）and the detection limit was 0.062μg/L. Different concentrations of ricin,abrin,staphylococcal enterotoxin B had almost no interference on the test results ,The results had good reproducibility.

关 键 词：磁性纳米粒子 功能化 β-银环蛇毒素 检测 

分 类 号：Q441.2[生物学—生理学]