白假丝酵母菌假菌丝标本片的制作方法  

The method of specimen slice production of Candidaalbicans' s pseudohyphae

作　　者：盖红雷 宋苑萌 张维嘉[1] 宋富成[1] 吕锐[1] 张文卿[1] 王斌[1] 

机构地区：[1]青岛大学医学院,山东青岛266071

出　　处：《青岛医药卫生》2013年第6期407-408,共2页Qingdao Medical Journal

基　　金：青岛大学医学院2012年度本科生创新实验项目支持

摘　　要：目的 探讨白假丝酵母菌假菌丝的形成条件,制作成标本片,用于病原微生物学实验教学.方法 将白假丝酵母菌接种于改良Sabourand＇s培养基,置37℃真菌培养箱孵育1～2天,然后转种于改良1% Tween-80玉米粉培养基继续培养3～4天,制成标本片,染色,显微镜下观察.结果 假菌丝形成良好,形态完整.结论 该方法培养的白假丝酵母菌假菌丝阳性率高、形态完整、易于观察、效果较好.Abstract：Objective To discuss the formation condition of Candida albicans＇s pseudohyphae and make into specimen slices for the experimental teaching of pathogeny microbiology. Methods Candida albicans was incubated into the medium of the improved Sabourands and put in 37℃ fungus incubator for 1-2 days, then transplanted into the medium of the improved 1 Tween-80 corn meal for further incubation for 3-4 days. Specimen slices were made, dyed and observed un der microscope. Results Pseudohypha formed well and was in complete form. Conclusion This effective way shows a high positive rate. Candida albicans s pseudohyphae cultured by this method is in a complete form and is easy to observe.

关 键 词：白假丝酵母菌 假菌丝 标本片制作 

分 类 号：R379.4[医药卫生—病原生物学]