猪繁殖与呼吸综合征病毒HH08株GP3蛋白的原核表达及其多抗血清的制备与检测  

Prokaryotic expression of porcine reproductive and respiratory syndrome virus HH08 strain GP3 protein and preparation and test of rabbit serum

作　　者：任晓峰[1] 刘和[1] 

机构地区：[1]东北农业大学动物医学学院,哈尔滨150030

出　　处：《东北农业大学学报》2013年第12期1-5,共5页Journal of Northeast Agricultural University

基　　金：教育部新世纪优秀人才基金资助项目(NCET-10-0144);教育部科技研究重点项目(212038);黑龙江省普通高等学校长江学者后备支持计划;教育部高等学校博士学科点专项科研基金(博导类)(20122325110019);哈尔滨市科技创新人才研究专项资金项目(RC2012XK002003)

摘　　要：GP3蛋白是猪繁殖与呼吸综合征病毒次要结构蛋白之一,是由ORF3基因编码一种高度糖基化糖蛋白,在病毒致病性、复制、装配、变异和保护性反应等方面具有重要意义。目前对GP3蛋白研究仍不透彻,在病毒感染细胞过程中所起作用也不清楚。试验扩增PRRSV-HH08株ORF3,连接至PGEX-6P-1载体,导入大肠杆菌内诱导表达,获得以包涵体存在GP3蛋白,纯化后作为抗原制备兔抗GP3多抗血清,Western-blotting结果表明,该蛋白与多抗血清有免疫反应,鉴于ORF3与ORF5基因保守度极低,可用于与其他蓝耳病毒株GP3或GP5蛋白为抗原制作多抗血清比较敏感性及特异性。GP3 protein is one of the minor structure proteins of Porcine Reproductive and Respiratory Syndrome Virus, is a highly glycosylated protein coded by ORF3, which plays a role key in pathogenicity, replication, assembling, mutation and protective reaction of the virus. However, at present the research on GP3 protein is still not clear enough, the function in infection is still unknown. This experiment amplified the ORF3 of the PRRSV strain HH08, linked to PGEX - 6P-1 carrier, imported into e. coil and induced its expression, obtained the inclusion body existing GP3 protein, injected into rabbit as antigen to produce GP3-polyclonal antibody serum after its purification. Western-blotting results proved that the GP3 protein had immune reaction.

关 键 词：PRRSV GP3 抗血清 

分 类 号：S828[农业科学—畜牧学] S851[农业科学—畜牧兽医]