“乳菌宝”益生菌剂对断奶仔猪肠道生理生化指标的影响  

Effect of “Rujunbao” probiotics on physiological and biochemical indexes of weaned piglets intestinal

作　　者：王华[1,2] 张淑梅[1] 钟颖婷 林标声[1,2] 沈绍新[1,2] 邱丰艳[1] 

机构地区：[1]龙岩学院生命科学学院,福建龙岩364012 [2]福建省预防医学与兽医生物技术重点实验室,福建龙岩364012

出　　处：《东北农业大学学报》2013年第12期27-31,共5页Journal of Northeast Agricultural University

基　　金：福建省教育厅A类项目(JA13309);福建省教育厅B类项目(JB13188);龙岩学院校内服务海西项目(LYXY2013)

摘　　要：试验采用120头健康状况良好的杜长大三元杂交仔猪,随机分为4组,每组10头,3个重复,试验时间28 d,试验结束后收集不同部位的肠道内容物和粪便用于生理生化指标及微生物数量测定。结果表明,基础日粮中添加0.15%益生菌剂即可促进仔猪生长。与对照组组比,添加0.5%、0.1%、0.15%三个不同浓度益生菌剂的试验组(Ⅰ、Ⅱ、Ⅲ组),其肠道氨浓度及pH下降显著(P<0.05),十二指肠、空肠的淀粉酶、总蛋白酶活性提高显著(P<0.05),但回肠的淀粉酶、总蛋白酶活性提高不显著(P>0.05)。3个试验组的肠绒毛高度均比对照组提高,其中试验组Ⅱ、Ⅲ组差异达到显著水平(P<0.05)。3个试验组的盲肠、回肠、粪便的致病菌数量均比对照组降低、有益菌数量增多,其中试验组Ⅲ组各微生物数量差异均达到显著水平(P<0.05)。3个试验组的腹泻指数均比对照组显著降低(P<0.05)。益生菌剂能提高仔猪采食量、促进生长的机理为其降低仔猪肠道氨浓度及pH,提高仔猪肠道消化酶活性,改善仔猪肠道微生物区系平衡、增加有益菌数量、抑制有害致病菌生长,提高仔猪肠道健康、减少腹泻。All of 120 Duroc × Landrace × Yorkshire crossbred weaned piglets, in good physical condition, were randomly housed in four groups with 3 pens of 10 each and to feed for 28 d. After experiment, the intestinal contents from different parts and dejecta were collected to determine the physiological and biochemical indexes and microbial population. The results showed that supplementation with basal diet ＋ 0.15% probiotics can better promote the growth of piglets. Compared with the control group, the ammonia concentration and pH value of three test groups （the I, II, III group） were fed the basal diet supplemented with the probiotics at ration of 0.5%, 0.1% and 0.2% decreased and the amylase and total protease activity of duodenum and jejunum increased significantly （P〈O.O5）,while the amylase and total protease activity of ileum increased insignificant（P〉O.05）. Moreover, the intestinal villus height of three test groups increased, and the different between the test group（ll, III group） and control group reached significant level（P〈O.05）. The number of harmful bacteda of cecum, ileum and dejecta from three test groups decreased and beneficial bacteria increased, and the different between the test III group and control group reached significant level（P〈O.05）. Diarrhea index of three test groups were significantly lower than control group. In conclusion, the probiotics can improve the feed intake and enhance growth performance of piglets as a result of it can reduce the intestinal ammonia concentration, pH value and improve the digestive enzyme activity in intestinal tract. The probiotics also could improve the balance of intestinal flora through increasing the number of beneficial bacteria and inhibiting the harmful bacteria growth. In addition, the probiotice could improve intestinal health to reduce diarrhea.

关 键 词：益生菌剂 断奶仔猪 肠道 生理生化指标 

分 类 号：S816-3[农业科学—饲料科学] S83[农业科学—畜牧学]