中药复方对急性热应激昆明鼠血钙水平及肺、肾脏钙含量的影响  

Effect of compound Chinese herbal medicine on blood calcium level and concentration of Ca^(2+) in lung and kidney tissues of mouse under acute heat stress

作　　者：郭世宁[1] 段凯文[2] 严凌苓[2] 郭晓华[2] 欧阳清芳[2] 石达友[1] 

机构地区：[1]华南农业大学兽医学院,广州510642 [2]江西生物科技职业学院,南昌330200

出　　处：《东北农业大学学报》2013年第12期89-92,共4页Journal of Northeast Agricultural University

基　　金："十一五"国家科技支撑计划项目(2008BAQB4B01-6);广东省教育部产学研结合项目(2008B090500250);广东省中国科学院全面战略合作项目(2010B090301040);广东省教育部产学研合作项目(2012B091100482)

摘　　要：选用体质健康,体重毫无显著差异的6~7周龄健康SPF级昆明鼠70只,并将其随机分为复方Ⅰ、复方Ⅱ和复方Ⅲ、紫锥菊组、王老吉组、阳性空白对照组,阴性空白对照组,共7组,每组10只。本试验在第1 h、3 h,每组随机取5只昆明鼠,检测急性热应激昆明鼠血清和肺、肾脏组织钙离子浓度(Ca2+)。结果表明,热应激下,小鼠血钙水平下降,肺、肾组织钙含量下降,而中药复方组能有效提高热应激昆明鼠血钙水平并能降低肺组织、肾组织钙浓度下降幅度,复方Ⅲ和紫锥菊能显著提高热应激小鼠血钙水平,复方Ⅰ对维持肺脏钙浓度作用明显。本试验结果显示,中药可能通过提高血钙水平的作用从而提高机体抗热应激能力。The aim of the study was to prove whether or not the compound Chinese herbal medicine has effects on the blood calcium level and concentration of calcium （Ca^2＋） in mouse lung and kidney Tissues in vivo in a model of acute heat stress. Seventy 6-7 week-age health SPF Kunming mouse whose weight were no significant different were randomly divided into seven groups： compound Chinese herbal medicinel group （groupl）, compound Chinese herbal medicinell group （groull）, compound Chinese herbal medicine III group （group III）, Echinacea group, Wanglaoji group, positive control group, negative control group. Five mouse were randomly chose at 1 and 3 h. The blood calcium level and Ca^2＋ in lungs and kidney tissues of all groups were detected by calcium kit. The results showed that blood calcium levels were decreased as well as the Ca^2＋ in lung and kidney of mouse under acute heat stress, while the traditional Chinese medicine group can effectively improve the blood calcium level of mouse under acute heat stress, however, the Ca^2＋ in lung and kidney could be decreased at 3 h, while which could be attenuated by compound Chinese herbal medicine and wanglaoji. The blood calcium level were significantly increased by compound Chinese herbal medicine III and Echinacea purpurea. Compound Chinese herbal medicinel was capable of maintainning the level of calcium concentration in the lungs. The test results suggested that traditional Chinese medicine were capable of protecting animals from acute heat stress by raising blood calcium level.

关 键 词：中药 钙离子 热应激 昆明鼠 

分 类 号：S853.5[农业科学—临床兽医学]