类风湿性关节炎患者血清一氧化氮及内皮素-1水平检测  

Determination of nitric oxide and endothelin-1level in Rheumatoid arthritis patients serum

作　　者：刘莲[1] 蒋红梅[1] 费樱[1] 周清[1] 

机构地区：[1]贵阳医学院检验系,贵州贵阳550004

出　　处：《中国现代医学杂志》2013年第31期32-34,共3页China Journal of Modern Medicine

基　　金：国家级教学团队资金(No:教高函(2009)18号);贵州省科技厅贵阳医学院联合基金(No:黔科合LG字[2012]004号

摘　　要：目的探讨类风湿性关节炎(rheumatoid arthritis,RA)患者血清一氧化氮(nitric oxide,NO)和内皮素-1(endothelin-1,ET-1)水平的变化情况。方法以55例RA患者和29例健康志愿者为研究对象,用硝酸还原酶法和生物素双抗体夹心酶联免疫吸附法(ELISA)检测血清中NO及ET-1的水平,结合血清类风湿因子(rheumatoid factors,RF)和抗环胍氨酸肽(cyclic citrullinated peptide,CCP)进行分析。结果 RA患者血清NO水平明显高于健康对照组(P<0.05);两组间血清ET-1水平差异无显著性;RF阳性与RF阴性的RA患者之间血清NO、ET-1水平无明显差异,但抗CCP抗体阳性RA病人血清NO水平显著高于抗CCP抗体阴性者。结论 NO可能在RA的发生发展过程中起着一定作用。【Objective】To studychanges of the levels of serum nitric oxide（NO） and endothelin-1（ET-1） in rheumatoid arthritis（RA） patients.【Methods】55 cases of patients with RA and 29 cases of healthy volunteers were used as the research subjects, nitrate reduction method and biotin double antibody sandwich enzyme-linked immunosorbent assay（ELISA） were used to detect the levels of serum NO and ET-1, combined with serum rheumatoid factors（RF） and ring resistance guanidine peptide amino acid cyclic citrullinated peptide（CCP） were analyzed.【Re- sults】RA patients serum NO level was significantly higher than the healthy control group（P 0.05）. Serum levels of ET-1 had no significant difference between the two groups. Serum level of NO and ET-1 had no obvious difference between RF positive and RF negative patients with RA. But, Serum level of NO in the Anti CCP antibody positive RA patients was significantly higher than in the Anti CCP antibody negative RA patients. 【Conclusion】NO played a certain role in the development process at the RA.

关 键 词：类风湿性关节炎 一氧化氮 内皮素-1 

分 类 号：R593.22[医药卫生—内科学]