冬虫夏草及人工虫草抗肝纤维化作用谱效关系研究  

Spectrum-effect relationship on anti-hepatic fibrosis efficacy of Cordyceps sinesis and its cultured mycelia

作　　者：吴素体[1] 白金霞[1,2] 王冶[1] 戴领[1,2] 吕青远[1] 于超[1] 韩晋[1] 袁海龙[1] 

机构地区：[1]中国人民解放军第三〇二医院药学部,北京100039 [2]成都中医药大学药学院

出　　处：《环球中医药》2013年第11期801-805,共5页Global Traditional Chinese Medicine

基　　金：国家科技重大专项"重大新药创制"专项(2011ZX09201-201-14)(2011ZX09201-201-35);国家自然科学基金(81073069)

摘　　要：目的研究冬虫夏草及人工虫草的高效液相色谱指纹图谱与其抗肝纤维化药效的相关性,以揭示其抗肝纤维化作用的"药效成分组"。方法考察虫草高效液相色谱指纹图谱较优的测定分析方法,通过该HPLC测定分析法得到冬虫夏草、发酵虫草菌粉、百令胶囊及蛹虫草菌粉胶囊的指纹图谱,找出特征共有峰,以LX-2肝星状细胞系为模型研究其对肝纤细胞的抑制效果,用pearson相关分析研究谱效相关性。结果冬虫夏草、发酵虫草菌粉、百令胶囊及蛹虫草菌粉胶囊的高效液相色谱指纹图谱中共有10个特征共有峰,其中4、5、10号峰与肝星状细胞(LX-2)细胞抑制效果(OD值)相关性最好。结论冬虫夏草、发酵虫草菌粉及蛹虫草菌粉胶囊对LX-2细胞均有抑制效果,冬虫夏草及人工虫草HPLC指纹图谱与其抗肝纤维化作用之间有一定对应关系,可以作为目前冬虫夏草及人工虫草抗肝纤维化作用的质量控制的有效方法。Objective To investigate the relationship between HPLC of Cordyceps sinesis and its cultured mycelia and anti-hepatic fibrosis efficacy, in order to establish ＂active component group for reducing phlegm＂. Methods HPLC was adopted to determine and analyze HPLC fingerprints of Cordyceps sinesis and its cultured mycelia, then identify the characteristic peaks. Use the LX-2 hepatic stellate cells as a hepatic fibrosis model of Cordyceps sinesis and its cultured mycelia on the inhibitory effect. Then the cor- relation analysis was used to study the spectrum-effect relationship. Results The anti-hepatic fibrosis effi- cacy of Cordyceps sinesis and its cultured mycelia were resulted from the combined effect of a variety of in- gredients, it was tentatively concluded that peaks 4,5,10 related best to LX-2 cells inhibitory effect-OD value in 10 characteristic peaks. Conculusion Cordyceps sinesis and its cuhured mycelia can inhibit LX- 2 hepatic stellate cell proliferation in some extent. There may be certain relationship between their HPLC fingerprint and anti-hepatic fibrosis efficacy. This research can be used as the quality control method of Cordyeeps sinesis and its cultured myeelia when they were used for liver fibrosis.

关 键 词：冬虫夏草 人工虫草 指纹图谱 抗肝纤作用 相关分析 谱效关系 

分 类 号：R285.5[医药卫生—中药学]