RT-PCR法分析两种解表法中药对FM_1感染小鼠肺中炎性细胞因子的影响  

Influence of the lung inflammatory cytokines in model rats infected with influenza virus FM_1 by treating with two kinds of Chinese medicine compounds for reliving exterior syndromes through RT-PCR method

作　　者：徐红日[1] 王成祥[2] 王兰[1] 王惠芳[3] 张靖[3] 殷人易[4] 姜良铎[2] 周平安[5] 

机构地区：[1]北京中医药大学东直门医院急诊科,100700 [2]北京中医药大学东直门医院内科,100700 [3]中国人民解放军防化研究院 [4]武汉市第一医院呼吸科 [5]北京中医药大学东方医院内科

出　　处：《环球中医药》2013年第11期805-810,共6页Global Traditional Chinese Medicine

基　　金：国家自然科学基金(30873254)

摘　　要：目的 动态观察益气清瘟解毒合剂所含辛温解表法和辛凉解表法中药对流感病毒亚洲甲型鼠肺适应株（FM1）感染小鼠肺中炎性细胞因子表达的影响,探讨上述两种解表法抗流感免疫炎性损伤及其修复方面的作用机制.方法 建立FM1感染小鼠模型,将小鼠随机分为4组,分别为正常空白组、病毒感染模型组、辛温解表法组（炙麻黄6 g、羌活12 g、紫苏叶10 g）、辛凉解表法组（柴胡10 g、金银花10 g、薄荷6 g）,各组按病毒感染后第1、3、5、7天不同时相各分为4个小组,每小组8只小鼠.采用反转录酶聚合酶链式反应（reverse transcriptase polymerase chain veaction,RT-PCR）法,在流感病毒感染后不同时相,动态观察辛温、辛凉解表法中药对小鼠肺中TNF-α、IL- 6、IL-1、IL-10、IFN-γ 5种炎性细胞因子RNA表达的影响.结果 （1）辛温解表法中药在FM1感染小鼠后1～5天显著降低肺组织中TNF-α的RNA表达,在FM1感染后3～5天显著降低肺组织中IL-1的RNA表达,在FM1感染后第1天及第7天显著降低肺组织中IL- 6的RNA表达,在FM1感染后第3天及第7天,显著增加肺组织中抗炎性细胞因子IL-10的RNA表达,在FM1感染后1～3天显著增加抗病毒因子IFN-γ的RNA表达.（2）辛凉解表法中药在FM1感染小鼠后3～5天显著降低肺组织中TNF-α的RNA表达,在FM1感染后第7天显著降低肺组织中IL-1的RNA表达,在FM1感染后1～7天显著降低肺组织中IL- 6的RNA表达,在FM1感染后第3天及第7天,显著增加肺组织中抗炎性细胞因子IL-10的RNA表达,在FM1感染后1～3天显著增加抗病毒因子IFN-γ的RNA表达.在FM1感染后第3天辛凉解表法中药可能增加肺组织中IL-1的RNA表达.结论 中药可能通过纠正炎性细胞因子的失衡抑制机体的炎性损伤,并促进损伤的修复,对靶器官具有免疫保护作用.Objective To observe the influence of the lung inflammatory cytokines expression in model rats infected with influenza virus FM1 by treating with two kinds of Chinese medicine compounds for reliving exterior syndromes （Including relieving exterior with warm-pungent herbs and cold-pungent herbs in Chinese medicine theories）. To discuss the inflammation damage and repairing mechanism of these two compounds. Methods Model rats infected with influenza virus FM1 were divided into blank group, model control group, warm-pungent herbs group （ consists of Ephedra, Notopterygium and Purple Perilla） and cold-pungent herb group （consists of Radix Bupleuri, Honeysuckle and Mentha Haplocalyx） at random. Each group was divided by the different stages of infection in 1, 3, 5 and 7 day（8 for each）. Then observe the influence of TNF-ot, IL-6, IL-1, IL-10 and IFN-γ in model rats lungs in each group. Results The RNA expression in model rat lungs of TNF-α in 1 - 5 day levels, IL-1 in 3 - 5 day levels and IL-6 in 1,7 day levels were significantly reduced in warm-pungent herb group, while the expression of IL-10 in 3, 7 day levels and IFN-γ in 1 - 3 day levels were significantly increased. In cold-pungent herb group, the expression of TNF-α in 3 - 5 day levels, IL-1 in 7 day level and IL-6 in 1 - 7 day levels were significantly reduced, while the expression of IL-10 in 3,7 day levels and IFN-γ in 1 - 3 day levels were significantly increased, but the expression of IL-1 in 3 day level was significantly increased. Conelusion Diaphoresis Chinese medicine compounds for reliving exterior syndromes based on those two relieving exterior methods might be able to decrease inflammatory damages and enhance the recovery of injured by correcting inflam- matory cytokine imbalance.

关 键 词：反转录酶-聚合酶链式反应 解表法中药 流感病毒亚洲甲型鼠肺适应株(FM1) 小鼠 炎性细胞因子 

分 类 号：R285.5[医药卫生—中药学]