楷木与四叶参组合物对水貂呕吐模型的作用  

Effect of composition of Codonopsis lanceolata Pistacia and Pistacia chinensis Bge on vomiting model of mink.

作　　者：李晓玲 丁波[2] 刘明[3] 

机构地区：[1]青岛市立医院急诊科,266001 [2]山东中医药大学基础医学院 [3]青岛崂山药用植物研究所

出　　处：《环球中医药》2013年第11期815-818,共4页Global Traditional Chinese Medicine

摘　　要：目的采用新型水貂呕吐模型研究楷木与四叶参提取物的组合物(楷组合物)对水貂呕吐模型的作用。方法 (1)雄性水貂30只随机分成5组:顺铂模型对照组、昂丹司琼组、楷组合物(楷木提取物与四叶参提取物按1∶1混合)小、中、大剂量组,各组分别给予生理盐水、昂丹司琼、楷组合物处理30分钟后给予腹腔注射顺铂,观察顺铂注射后6小时内水貂呕吐潜伏期和干呕、呕吐次数。(2)雄性水貂30只随机分成5组:1-phenylbiguanide hydrochloride(PBG)模型对照组、昂丹司琼组、楷组合物(楷木提取物与四叶参提取物按1∶1混合)小、中、大剂量组,各组分别给予生理盐水、昂丹司琼、楷组合物处理30分钟后给予腹腔注射PBG,观察PBG注射后6小时内水貂呕吐潜伏期和干呕、呕吐次数。(3)将顺铂预处理的水貂在用药24小时时处死,取其回肠组织用高效液相电化学检测法(HPLC-ECD)测定5-羟色胺(5-HT)含量,并观察楷组合物对顺铂引起的水貂回肠5-HT释放的影响。结果楷组合物对顺铂和PBG所致水貂呕吐行为有抑制作用,可延长呕吐潜伏期、减少干呕和呕吐次数(P<0.05)。水貂回肠HPLC结果示,顺铂可促进回肠5-HT释放,升高回肠内5-HT含量(P<0.01)。楷组合物对顺铂引起的水貂回肠5-HT释放有抑制作用,可降低其含量的升高(P<0.05)。结论楷组合物有止呕作用;对顺铂引起的水貂回肠5-HT释放有抑制作用,可降低其含量的升高;止呕机制涉及到外周和中枢的5-HT及5-羟色胺3(5-HT3)受体系统。Objective To investigate the antiemetic effect of the composition of Pistacia chinensis and Codonopsis lanceolata Benth. et Hook （ Jie Combination ） on acute emesis in the new vomiting model of minks. Methods （1） Minks were randomly divided into cisplatin group, cisplatin ＋ ondansetron group, cisplatin ＋ low-dose Combination group, cisplatin ＋ middle-dose Combination group and cisplatin ＋ high-dose Combination group. The antiemetie effect of drugs was investigated in the vomiting model of minks induced by cisplatin （7.5mg · kg^-1 , i. p. ） in acute period （6 h） observation. （2） Minks were randomly divided into 1-phenylbiguanide hydrochloride （PBC） group, PBG ＋ ondansetron group, PBG ＋ low-dose Combination group , PBG ＋ middle-dose Combination group and PBG ＋ high-dose Combination group. The antiemetic effect of drugs was investigated in the vomiting model of minks induced by PBG （ 8 mg· kg^-1, i. p. ） in acute period （6 h） observation. （3） Cisplatin pretreatment of mink in 24 h, detected the content of 5- HT of the intestinal tissue by high performance liquid chromatography with electrochemical detection （HPLC-ECD） , and observe the effect of Jie composition on 5-HT releasing on mink ileum. Resuits The frequency of retching and vomiting was significantly reduced by pretreatment with Jie Combina- tion in middle and high dose （ P 〈 0. 05 ） . Jie composition inhibited the release of 5- HT on cisplatin induced mink ileal （ P 〈 0. 05 ）. Conclusion Jie Combination has a good activity against acute emesis in minks , the mechanism may involve 5-HT and 5-HT3 receptors of peripheral and central.

关 键 词：四叶参提取物 楷木提取物 水貂 呕吐 5-羟色胺3受体 

分 类 号：R285.5[医药卫生—中药学]