大鼠睾丸支持细胞对骨髓间充质干细胞免疫抑制作用的影响  

Effects of rat Sertoli cells on the immunosuppressive properties of bone marrow mesenchymal stem cells

作　　者：丁影[1] 邹天慧[1] 刘慧竹[1] 王喆歆[1] 傅慧超 蒋黎华[1] 李伟毅[1] 

机构地区：[1]上海交通大学医学院免疫学与微生物学系上海市免疫学研究所,200025

出　　处：《中华移植杂志（电子版）》2013年第3期21-24,共4页Chinese Journal of Transplantation(Electronic Edition)

基　　金：上海市卫生局科研计划课题资助项目(2009208);上海市重点学科建设项目上海市免疫学研究所基金(J50207)

摘　　要：目的探索睾丸支持细胞(Sertoli细胞)对骨髓间充质干细胞(BMSC)免疫抑制作用的影响,为二者在移植免疫中的联合应用提供思路。方法二步酶解法处理大鼠睾丸,分离Sertoli细胞;Percoll法分离大鼠BMSC;Ficoll法分离淋巴细胞;刀豆蛋白A(ConA)刺激进行T细胞转化试验;将Sertoli细胞、BMSC和Sertoli细胞+BMSC分别加入未经ConA处理的静止淋巴细胞培养体系和经ConA处理后的T细胞转化体系,MTT法测定淋巴细胞增殖情况,观察BMSC、Sertoli细胞或二者共培养对T细胞活化、增殖的影响。结果 BMSC、Sertoli细胞以及二者共培养对静止的淋巴细胞无明显作用。BMSC、Sertoli细胞及二者共培养对T细胞的活化、增殖均有明显的抑制作用,且Sertoli细胞与BMSC共培养时抑制作用呈现一定的协同性。结论 BMSC和Sertoli细胞均具有负性免疫调节作用,二者共培养可以进一步增强BMSC的免疫抑制效应。Objective To investigate the effects of Sertoli cells on the immunosuppressive activity of bone marrow mesenchymal stem cells （BMSCs）. Methods Sertoli cells were isolated using two steps enzyme digesting method from rat testicle. BMSCs were prepared using Percoll density gradient centrifugation method. Proliferation of lymphocytes induced by ConA was measured using MTY method. The effects of BMSCs and Sertoli cells on ConA-induced T lymphocyte transformation were investigated. Results BMSCs and Sertoli cells were shown to significantly inhibit T lymphocyte transformation induced by ConA. The inhibitory effects of BMSCs and Sertoli ceils co-culture system were stronger than that of BMSCs alone. Conclusions BMSCs and Sertoli cells exert immunosuppressive effects. Sertoli cells can improve the effects of BMSCs.

关 键 词：睾丸支持细胞 骨髓间充质干细胞 淋巴细胞 增殖试验 免疫抑制 大鼠 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]