甜菜褐斑病抗感种质细胞超微结构变化比较  

Comparison of Cellular Ultrastructure of Different Sugarbeet Germplasms Infected Cercospora beticola

作　　者：丁广洲[1,2] 李永刚[3] 赵春雷[1,2] 王希[1,2] 陈丽[1,2] 马凤鸣[3] 陈连江[1,2] 

机构地区：[1]黑龙江省普通高等学校甜菜遗传育种重点实验室,哈尔滨150080 [2]中国农业科学院甜菜研究所,哈尔滨150080 [3]东北农业大学农学院,哈尔滨150030

出　　处：《中国糖料》2014年第1期1-4,共4页Sugar Crops of China

基　　金：中国博士后科学基金项目(2012M520781)资助;国家甜菜产业技术体系建设项目(CARS-210101)资助

摘　　要：在褐斑病发病严重地块。对发病盛期甜菜抗感病种质资源叶片X光片影像及电镜显微结构进行了比较和分析，结果表明：感病种质叶片图像密度不均，整体影像出现泡状光影。受病原茵的侵蚀，感病种质表现为细胞壁变形、并发生质壁分离；叶绿体变形、片层结构零乱、有的膨胀扭曲并分解；线粒体膜破损，膜局部破裂，内舍物外流。出现大量的嗜锇颗粒，有的细胞器逐渐解体、膜系统遭到严重破坏，甚至整个细胞死亡。但抗病种质却明显不同，X衍射图像密度均匀．细胞结构相对完整。The X-ray image and uhrastructure change susceptible germplasm of sugarbeet in cercospora leaf spot that X-ray image density of susceptible germplasm leaf was of foliar cell were compared between resistant and nursery of continuous cultivation. The results showed not uniform, and the X-ray image presented bubbles, cytoderm infected by cercospora was partly deformed and cytoplasm was shrunk away from the cytoderm in the susceptible germplasm; chloroplast was distorted, lamellar structure was disordered, even some were inflated and decomposed; mitochondrial membrane was damaged and inclusion was overflowed. Osmiopilic globules increased obviously; organelle disintegrated gradually, membrane system was seriously destroyed, even entire cell was dead. But those of resistant germplasm were significantly different; the density of picture was even, cell structure was relatively intact.

关 键 词：甜菜褐斑病 抗感病种质 叶片细胞 超微结构变化 

分 类 号：S435.663[农业科学—农业昆虫与害虫防治]