结核分枝杆菌多克隆抗体在结核性病变中的诊断价值  

Diagnostic value of polyclonal antibody to mycobacterium tuberculosis in tuberculous

作　　者：薛丽京[1] 刘燕[1] 何桥[1] 谭耀驹[1] 

机构地区：[1]广州市胸科医院,广东广州510095

出　　处：《临床肺科杂志》2014年第1期69-72,共4页Journal of Clinical Pulmonary Medicine

摘　　要：目的探讨用结核分枝杆菌多克隆抗体检测组织中的结核分枝杆菌抗原对结核病的诊断价值。方法收集各部位不同类型结核性病变组织标本288例,非结核性病变组织标本81例,采用结核分枝杆菌多克隆抗体免疫组化染色标记检测组织中的结核分枝杆菌抗原,并与抗酸染色比较。结果结核组对结核分枝杆菌多克隆抗体呈阳性反应者234例,占81.25%,抗酸染色阳性者61例,占21.18%。阳性染色在结核病变组织中的干酪样坏死灶、郎汉氏巨细胞和上皮样细胞的胞质内出现鲜红色颗粒。渗出为主型的结核分枝杆菌多克隆抗体染色阳性率与变质为主型及增殖为主型相比均有统计学意义。结论采用结核分枝杆菌多克隆抗体免疫组化标记对结核病的诊断明显高于抗酸染色,有一定的鉴别诊断价值。Objective To study the value of immunohistochemical stain technique in the diagnosis of tuberculous by using polyclonal antibody to mycobacterium tuberculosis. Methods 288 cases of various types of tuberculous lesion and 81 cases with non-tuberculous lesion were collected. Mycobacterium tuberculosis antigen in the tissues was detected by polyclonal antibody to mycobacterium tuberculosis by the application of immunohistochemical technique,while comparing with acid-fast stain. Results 234 cases( 81. 25%) in the tuberculous group presented positive to polyclonal antibody to mycobacterium tuberculosis,and 61 cases( 21. 18%) presented positive acid-fast stain.The positive stain demonstrated bright red granules in Langhans giant cells and caseous necrotic tissue. The positive rate of polyclonal antibody to mycobacterium tuberculosis reaction to exudation type was significantly higher than those of the necrosis type and proliferation type. Conclusion The immunohistochemical technique using polyclonal antibody to mycobacterium tuberculosis is far more sensitive in diagnosis of tuberculosis than acid- fast stain does.

关 键 词：结核 免疫组织化学 结核分枝杆菌多克隆抗体 诊断 

分 类 号：R52[医药卫生—内科学]