壬基酚对大鼠F1子代脑基因表达的影响  

Effects of nonylphenol on brain gene expression profiles of F1 generation

作　　者：南楠[1] 詹平[1] 

机构地区：[1]重庆医科大学公共卫生与管理学院劳动卫生与环境卫生教研室,重庆400016

出　　处：《激光杂志》2014年第1期69-70,72,共3页Laser Journal

摘　　要：目的:研究壬基酚通过妊娠期暴露是否会影响F1子代的脑发育。方法:对SD孕鼠染毒NP,从妊娠第7天开始持续到产后第二天,分别提取NP组和对照组F1代2d龄大鼠的脑组织mRNA,表达谱基因芯片检测脑基因的表达。用Q-PCR法验证F1雄性子代大脑泛素特异性蛋白酶8、ATP结合盒转运子和内磺肽蛋白这三个下调基因的表达,然后用Western blotting法检测F1子代大脑泛素特异性蛋白酶8的蛋白表达。结果:ATP结合盒转运子(ATP-binding cassette,ABCA)、内璜肽蛋白(Endosulfine alph,Ensa)、泛素特异性蛋白酶8(Ubiquitin Specific Protease 8,USP8/UBPy)基因和蛋白表达下调。结论:NP可影响神经细胞的正常结构和信号转导功能,并提示可以干扰机体内分泌系统、脂质和能量代谢及一系列正常生理功能。Objective：To investigate the adverse effects of nonylphenol （NP） on the development of brain of the F1 male rats after gestation exposure.Methods： Pregnant rats were exposed to NP at doses of 0 and 200mg/kg body weights, respectively from pregnant days 7 to after pregnancy to 2 days post partum. The 2 days brain tissue of the F1 progeny of male rats were tak- en, mRNA were extracted. The RQ-PCR method and Western blotting were used to detect the expression of ubiquitin-specific pro- tease 8 protein and AW141129, BE096678. NM-021842 abnormal gene expression, and analysis of possible mechanisms.Re- suits： ATP-binding cassette transporter （ATP-binding cassette,ABCA）, a-Endosulfine protein （Endosulfine alpha,Ensa） , ubiqui- tin-specific protease 8 （USP8 / UBPy） gene and protein expression decreased;.Conclusion：Nonylphenol can effect the structure of neurocyte and function of signal transduction.NP can interfere with neuroendocrine system function, affect lipid and energy metab- olism and a series of normal physiological function.

关 键 词：壬基酚 大鼠F1子代 基因表达 蛋白表达 

分 类 号：TN244.8[电子电信—物理电子学]