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作 者:黄继光[1] 杨文杰[1] 桑晓清[1] 赵欢欢[1]
机构地区:[1]华南农业大学资源环境学院/天然农药与化学生物学教育部重点实验室,广东广州510642
出 处:《华南农业大学学报》2014年第1期64-68,共5页Journal of South China Agricultural University
基 金:国家自然科学基金(31000868)
摘 要:【目的】在前期研究基础上,系统评价羽裂蟹甲草Cacalia tangutica的杀虫活性.【方法】采用触杀、胃毒和点滴等方法测定了羽裂蟹甲草中9种化学成分对家蝇Musca domestica、致倦库蚊Culex pipiens quinquefasciatus、菜粉蝶Pieris rapae、亚洲玉米螟Ostrinia furnacalis、红火蚁Solenopsis invicta的生物活性.【结果和结论】无羁萜对家蝇3龄幼虫的触杀活性明显,1μg/头点滴处理后48 h,校正死亡率达到86.18%,与鱼藤酮(85.67%)无显著差异.无羁萜和山奈酚对致倦库蚊4龄幼虫的毒杀活性与鱼藤酮不显著,处理后72 h,校正死亡率均超过80%.此外,无羁萜对菜粉蝶3龄幼虫、亚洲玉米螟4龄幼虫、红火蚁表现出了一定的触杀活性,无羁萜、槲皮素和山奈酚对红火蚁还具有较明显的胃毒活性.结果表明无羁萜、槲皮素、山奈酚是羽裂蟹甲草中的活性成分.[ Objective ] To assess the insecticidal activities of Cacalia tangutica based on previous resear- ches. [ Method ] The activities of several compounds from Cacalia tangutica against Musca domestica, Culex pipiens quinquefasciatus, Pieris rapae, Ostrinia furnacalis, Solenopsis invicta were bioassayed by contact, stomach poison and drop treatment. [ Result and conclusion ] The results indicated that friedelin possessed a strong contact toxicity against third-instar larvae of M. domestica, and the corrected mortality was 86. 18% after treatment for 48 h with 1 p,g each larva by topical application, which was not signifi- cantly different from that of rotenone(85.67% ). Friedelin, kaempferol and rotenone were equitoxic to fourth-instar larvae of C. pipiens quinquefasciatus. The corrected mortalities after treatment for 72 h were more than 80%. Moreover, friedelin had some degree of contact toxicities against third-instar larvae of P. rapae, fourth-instar larvae of O. furnacalis and S. invicta. Friedelin, quercetin and kaempferol had obvious stomach poison activities to S. invicta. Generally friedelin, quercetin and kaempferol are the ac- tive ingredients of C. tangutica.
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