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作 者:张姿丽[1] 蒋锋[1] 刘鹏飞[1] 陈青春[1] 张媛[1] 王晓明[1]
机构地区:[1]仲恺农业工程学院作物研究所,广东广州510225
出 处:《华南农业大学学报》2014年第1期110-113,共4页Journal of South China Agricultural University
基 金:公益性行业(农业)科研项目(201303008);广东省科技计划项目(2012B020301006)
摘 要:【目的】为改良玉米雄穗性状.【方法】以雄穗一级分枝数有显著差异的超甜玉米自交系T4和T19为亲本,构建了包含232个单株的F:群体,考察雄穗一级分枝数,利用复合区间作图法进行QTL定位.【结果和结论】结果获得一张包含77个SSR标记的遗传连锁图谱,全长868.7cM,标记平均间距为11.28cM,共检测到4个与超甜玉米雄穗一级分枝数相关的QTL位点,分别位于玉米第4、7、8染色体上,可解释5.08%~17.71%的表型变异.主效QTL位点q船Ⅳ-4位于第8染色体,可解释17.71%的表型变异.这些QTLs将为雄穗一级分枝数的分子标记辅助选捧提供依据.[ Objective ] To improve the tassel of maize. [ Method ] In this study, a cross between sweet corns inbred T4 and T19, which were significantly different in tassel primary branch number, was de- signed and QTLs for tassel primary branch number were identified in a whole genome with the methods of multiple interval mapping (CIM) based on 232 F2 individuals. [ Result and conclusion ] A genetic link- age map composing of 77 simple sequence repeat (SSR) markers covering 868.7 cM with an average in- terval of 11.28 cM was constructed. Four QTLs for tassel primary branch number in chromosome 4, 7, 8 were detected, accounting for 5.08% - 17.71% of phenotypic variance. The main QTLs were located on chromosome 8 which accounted for 17.71% of the total phenotypic variance. These QTLs provide the ba- sis of marker-assisted selection for tassel primary branch number.
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