产表面活性剂石油降解菌株的筛选及鉴定  

The screening and identification of oil degradation bacteria producing surfactant

作　　者：花莉[1] 洛晶晶[1] 胡阳阳[1] 彭香玉[1] 贾卫华[2] 

机构地区：[1]陕西科技大学资源与环境学院,陕西西安710021 [2]国家林业局林产工业规划设计院,北京100010

出　　处：《陕西科技大学学报（自然科学版）》2014年第1期34-38,共5页Journal of Shaanxi University of Science & Technology

基　　金：国家自然科学基金项目(41003042);陕西省科技厅自然科学基金项目(2011JQ5001);陕西省教育厅科学研究计划项目(2013JK0890);陕西科技大学研究生创新基金项目

摘　　要：从长期受油污的土壤中分离得到了7株降解石油类菌株,其编号分别为菌2-1、菌7-1、菌1-2、菌5-2、菌7-2、菌油3及菌油5.经形态观察、Biolog鉴定和16SrDNA基因序列分析,可鉴定菌2-1和菌7-1为粘质沙雷氏菌,菌1-2为居植物柔武氏菌,菌5-2、菌油3和菌油5都为克雷伯氏菌属,菌7-2为蜡状芽孢杆菌.其中,菌1-2、菌5-2和菌7-2能使发酵液的表面张力从36.10mN/m降低至20.20mN/m、20.74mN/m、21.78mN/m,表明这些菌所产生的表面活性剂能具有较强的乳化原油的能力,展现了较大的应用前景.7 strains degrading oil were isolated from long-term oil-contaminated soil and they were numbered as bacteria 2-1 ,bacteria 7-1 ,bacteria 1-2 ,bacteria5-2 ,bacteria 7-2 ,bacteria oil 3 ,bacteria oil 5 ,of which bacteria 2-1 and bacteria 7-1 were identified as Serratia marces-cens ,bacteria 1-2 as Raoultella planticola ,bacteria 5-2 ,bacteria oil 3 ,and bacteria oil 5 as Klebsiella genus ,bacteria 7-2 as Bacillus cereus by morphological observation ,Biolog identi-fication and 16S rDNA gene sequence analysis .Bacteria 1-2 ,bacteria 5-2 and bacteria 7-2 en-abled the surface tension of the fermentation liquid reduce from 36 .1 mN /m to 20 .2 mN /m ,20 .74 mN /m and 21 .78 mN/m ,indicating the surfactants produced by the bacteriums have a strong emulsification crude capacity and show great potential .

关 键 词：石油污染 产表面活性剂微生物 筛选 鉴定 

分 类 号：X53[环境科学与工程—环境工程]