人参皂苷对肿瘤坏死因子-α致心肌肥大抑制作用  

Inhibitive effects of ginsenoside Rg1 on cardiomyocyte hypertrophy induced by tumor necrosis factor-α

作　　者：王琰[1] 张佩[1] 梁灵君[2] 孙雪芳[2] 王洪新[2] 

机构地区：[1]辽宁医学院免疫学研究室,辽宁锦州121001 [2]辽宁医学院药理学教研室

出　　处：《中国公共卫生》2014年第1期77-79,共3页Chinese Journal of Public Health

基　　金：辽宁省自然科学基金(201102141)

摘　　要：目的探讨人参皂苷Rg1对肿瘤坏死因子-α(TNF-α)诱导的乳鼠心肌细胞肥大保护作用及机制。方法以原代培养乳鼠心肌细胞为模型,应用TNF-α100μg/L诱导心肌细胞肥大,观察不同浓度人参皂苷Rg1对心肌肥大影响;用Lowry法测定心肌细胞蛋白含量,逆转录-聚合酶链反应(RT-PCR)法检测心肌细胞心房钠尿肽(ANP)和Toll样受体4(TLR4)mRNA表达,Western blot法检测心肌细胞TLR4和抑制蛋白-κBα(IκBα)蛋白含量。结果与对照组比较,模型组细胞蛋白含量、ANP、TLR4 mRNA和蛋白表达均增加(P<0.01),IκBα蛋白表达减少(P<0.01);与模型组比较,30μmol/L人参皂苷心肌细胞蛋白含量[(19.76±1.09)μg]、ANP mRNA表达(0.609±0.001)、TLR4 mRNA含量(0.899±0.001)和TLR4蛋白含量(0.46±0.03)表达均降低(P<0.05);IκBα蛋白含量(0.70±0.09)升高(P<0.05);人参皂苷Rg1能有效抑制TNF-α诱导的心肌肥大。结论人参皂苷Rg1对TNF-α诱导的乳鼠心肌细胞肥大有保护作用,其机制可能与TLR4/核转录因子-kB(NF-kB)信号通路有关。Objective To investigate protective effects and mechanism of ginsenoside Rgl on cardiomyocyte hyper trohy induced by tumor necrosis factor-α（TNF-α） in neonatal rats. Methods The primary cultured of neonatal rat ven tricular myocytes were used and the hypertrophic cardiac myocytes were induced by TNF-α at the dose of 100 μg/L, and then the effect of different concentrations of ginsenoside Rgl on cardiac hypertrophy was observed. Total protein content was assayed with Lowery method ; the expressions of atrial natriuretic peptide （ANP） and Toll like receptor 4 （ TLR4 ） mRNA were determined with reverse transcription-polymerase chain reaction（RT-PCR） ;the expression of TLR4 and in hibitor-KBα （IKBa）was measured by western blot. Results Compared with those of the control group, protein content, ANP mRNA,and TLR4 mRNA and protein were significantly increased（P 〈0. 01 for all）and IKBa decreased in TNF-α group （P 〈 0. 01 ）. Compared with TNF-α group, ginsenoside Rgl of 30 μmol/L significantly inhibited TNF-α-induced cardiac hypertrophy and decreased the protein content （ 19.76 ± 1.09 μg） （P 〈 0. 05 ）and the expression of ANP mRNA （0. 609 ± 0. 001 ） （P 〈 0. 05 ）. Ginsenoside Rgl at dose of 30 μmol/L could also significantly alleviate the inflammatory response induced by TNF-α, which may be partially via attenuating IKBa （ 0. 70 ± 0. 09 ） （ P 〈 0. 05 ） and the high expres sion of TLR4 mRNA （0. 899 ± 0. 001 ） and protein （0. 46 ± 0. 03 ） induced by TNF-α. Conclusion Ginsenoside Rgl has protective effect on TNF-α-induced cardiac hypertrophy. The mechanism may be related to the inhibition of TLR4/NF-KB.

关 键 词：人参皂苷RG1 肿瘤坏死因子-α(TNF-α) 心肌肥大 Toll样受体4(TLR4) 核转录因子(NF-kB) 

分 类 号：R285.5[医药卫生—中药学] R541[医药卫生—中医学]