普通小麦细胞质雄性不育保持系mtDNA基因文库的构建  

Construction of mtDNA Library of CMS Maintainer of Common Wheat

作　　者：赵宝存[1] 黄占景[1] 沈银柱[1] 何聪芬[1] 秘彩莉[1] 刘植义[1] 

机构地区：[1]河北师范大学生物系,河北石家庄050016

出　　处：《华北农学报》2000年第4期45-51,共7页Acta Agriculturae Boreali-Sinica

基　　金：河北省自然科学基金资助项目! (30 0 170 )

摘　　要：以普通小麦细胞质雄性不育保持系 75 3369B为材料 ,分别用限制性内切酶EcoRⅠ和MboⅠ对 75 3369B的mtDNA进行完全酶切和部分酶切 ,构建了两个基因文库。其中用EcoRⅠ酶切得到的库 (以下简称ME库 )由 350个重组子构成 ;MboⅠ酶切所构建的库 (以下简称MM库 )由 4 50个重组子构成 ,MM库的库容量是 98 12 %。同时 。Cytoplasmic male sterility(CMS)line 75-3369A's maintainer 75-3369B of common wheat was studied.mtDNA was digested completely with EcoRⅠ and partly with MboⅠ and two gene libraries(ME and MM)of mitochondrial DNA(mtDNA)of 75-3369B was constructed.ME consisted of 350 clones and MM contained 450 clones.MM library included 98 12 percent of the mitochondrial genome.The advantage and disadvantage of the two methods of gene library construction were also discussed.

关 键 词：小麦 保持系 MTDNA 基因文库 细胞质雄性不育系 

分 类 号：S512.103.5[农业科学—作物学] Q785[生物学—分子生物学]