绿盲蝽对寄主转换的适应性及生理响应  

Adaptability and Physiological Response to Host Plant Species Switching in Apolygus lucorum

作　　者：肖留斌[1] 谭永安[1] 孙洋[1] 赵洪霞[1] 吴国强[1] 柏立新[1] 

机构地区：[1]江苏省农业科学院植物保护研究所,南京210014

出　　处：《中国农业科学》2013年第23期4941-4949,共9页Scientia Agricultura Sinica

基　　金：国家公益性行业(农业)科研专项(201103012);国家现代农业产业技术体系建设专项资金(CARS-18-16)

摘　　要：【目的】明确绿盲蝽在不同寄主植物之间转换后的适应性与生理响应机制。【方法】采用昆虫试验种群生命表方法,系统测定用四季豆饲养2代后的绿盲蝽分别转换到豇豆、绿豆、鄂杂棉10号(Bt棉)和苏棉9号(非Bt棉)上的种群生长发育与繁殖等生命表参数,并分析不同供试寄主饲养的绿盲蝽成虫体内的淀粉酶、蛋白酶及海藻糖酶活性和海藻糖含量。【结果】绿盲蝽由四季豆转移到不同供试寄主植物上均可完成世代循环,但不同龄期若虫的发育历期与存活率、5龄若虫体重及成虫寿命与产卵量等差异显著。不同供试寄主饲养的绿盲蝽种群趋势指数值依次为:四季豆(16.56)﹥Bt棉-鄂杂棉10号(3.23)﹥常规棉-苏棉9号(2.14)﹥豇豆(1.67)﹥绿豆(1.31)。不同供试寄主饲养的绿盲蝽成虫体内的几种酶活性也存在较大差异。转基因棉(鄂杂棉10号)种群的海藻糖酶活性最高(3.74μg·mg-1·min-1),而海藻糖含量最低(3.03μg/adult)。【结论】绿盲蝽由四季豆转换到其它4种寄主后,其生长发育速率与种群趋势增殖指数等生命参数均有所下降,成虫体内的蛋白酶、淀粉酶、海藻糖酶活性及海藻糖含量也有显著差异。总体相对比较而言,绿盲蝽对棉花具有较高的适应性。[Objective] The objective of this study is to clarify the effects of plant species switching on reproduction, growth and physiological responses of the experimental population of green blind bugs （Apolygus Lueorum）. [Method] The life tables of A. lucorum in a laboratory population were constructed to systematicly study the effect of different species hosts （Phaseolus vulgaris, Vigna unguiculata, E radiata, Sumian9, Ezamianl0）. The activities of trehalase, amylase and proteinase and the content of trehalase were examined in laboratory populations ofA. lucorum that reared on different species of host plants. [Result] The developmental periods, survival rates, body weight of 5th instar, longevity and hatching rate ofA. lucorum were significantly different among the five species host plants. Population increased from high to low was P vulgaris （16.56）〉Ezamian10 （3.23） 〉Sumian9 （2.14） 〉 V. unguiculata （1.67） 〉 I4. radiate （1.31） by comprehensive analysis of life tables. At the same time, there was a certain difference of the trehalase activity and the content of trehalose ofA. lucorum on different host plants. The trehalase activity for Ezamian 10 came to the peak was at 3.74 ~tg.mgq.min1 （P〈0.05）, however, the trehalose content came to the valley was at 3.03 ~tg/adult （P〈0.01）. [Conclusion] As green blind bugs on P vulgaris transferred to the other four species host plants, life parameters decreased, such as population trend index and so on. The frequency differences were obviously among these five populations. In general, A. lucorum has high adaptability to cotton.

关 键 词：绿盲蝽 寄主转换 生命参数 生理响应 

分 类 号：S433[农业科学—农业昆虫与害虫防治]