对虾白斑症病毒(WSSV)部分克隆片段的序列测定及PCR扩增  

Sequencing of Partial Genome DNA of Penaeid Shrimp White Spot Syndrome Virus (WSSV) and Its Amplification by PCR

作　　者：谢数涛[1] 何建国[1] 吕玲[1] 吴言云 江静波[1] 

机构地区：[1]中山大学生命科学学院

出　　处：《湛江海洋大学学报》2000年第3期22-26,共5页Journal of Zhanjiang Ocean University

摘　　要：对克隆的 WSSV基因组 Xba 1.8kb片段进行序列测定 ,根据所测定的核酸序列 ,通过计算机软件分析 ,设计出一对 PCR引物。所设计的 PCR引物能从纯化 WSSV及患白斑症的对虾组织中扩增出长为 92 1bp的目的 DNA片段 ,并且能检测出 0 .2～ 0 .4μg病虾肌肉组织中的 WSSV。健康对虾、感染MBV的斑节对虾仔虾及 SINPV的扩增结果均为阴性。表明所建立的 WSSV PCR检测法灵敏而且特异。A 1837 bp fragment of WSSV genome was sequenced.Based on this sequence,a pair of primers producing 921 bp amplified fragment was designed.The expected amplification product was obtained when templates were extracted from purified virions of WSSV or tissues of naturally diseased Penaeus monodon associating with white spot syndrome.The detection limit of diseased shrimp muscle was about 0.2～0.4 μg.There was no amplification product when templates were extracted from healthy penaeid shrimps,postlarvae of p. monodon infected with MBV,or virions of purified SINPV.So a sensitive,specific and rapid PCR has been provided for detecting WSSV.

关 键 词：白斑症病毒 斑节对虾 序列测定 PCR扩增 基因克隆 

分 类 号：S945.409[农业科学—水产养殖] Q78[农业科学—水产科学]