不同浓度氧化性低密度脂蛋白对成年大鼠心肌细胞的损伤作用  

Different concentration of ox-LDL on the damage of adult rat cardiomyocytes

作　　者：王洁[1] 刘颖[1] 王雷 宫海滨 

机构地区：[1]徐州市中心医院中心实验室,江苏省221009 [2]江苏省徐州市心血管病研究所

出　　处：《中华临床医师杂志（电子版）》2013年第18期123-125,共3页Chinese Journal of Clinicians(Electronic Edition)

基　　金：国家自然科学基金项目(30572073)

摘　　要：目的研究不同浓度氧化性低密度脂蛋白(ox-LDL)对成年大鼠心肌细胞的损伤作用。方法本实验采用铜氧化LDL法制备ox-LDL,分为1μg/ml、2.5μg/ml、5μg/ml、7.5μg/ml、10μg/ml5个浓度组和PBS对照组。LDL亦分为相应浓度组。测定培养的心肌细胞的存活率。结果与对照组相比,2.5μg/ml ox-LDL组心肌细胞培养24 h、5μg/ml ox-LDL组培养8 h、10μg/ml ox-LDL组培养4 h,心肌细胞存活率均明显降低(P<0.05)。LDL组与对照组相比心肌细胞存活率无明显差异,随着ox-LDL作用时间的延长,心肌细胞的存活率逐渐下降。结论 ox-LDL的浓度和作用的时间与心肌细胞损伤的程度有着密切的关系。Objective To investigate different concentration of ox-LDL on the damage of adult rat myocardial cell. Methods LDL was oxidized by method of copper oxide.Experiment was divided into 1μg/ml, 2.5μg/ml, 5μg/ml, 7.5μg/ml, 10μg/ml ox-LDL groups and PBS control group,and so was LDL. Survival rate was determined in cultured myocardial cells. Results Compared with control group, 2.5μg/ml ox-LDL significantly decreased the survival rate of cardiomyocytes when incubation for 24 h （P〈0.05）;5μg/ml ox-LDL significantly decreased the survival rate of cardiomyocytes when incubation for 8 h （P〈0.05）;10μg/ml ox-LDL significantly decreased the survival rate of cardiomyocytes when incubation for 4 h （P〈0.05）. There was no significantly difference between LDL group and control group. As the increasing of the concentration and the prolonging of time, the survival rate of myocardial cells became smaller. Conclusion The degree of damage to myocardial cells is related to both action time and action concentrations of ox-LDL.

关 键 词：脂蛋白类 LDL 肌细胞 心脏 存活率 

分 类 号：R543.5[医药卫生—心血管疾病]