塞来昔布对骨关节炎大鼠疼痛评分和SP表达的影响  

The role of celecoxib to pain scores and substance P in osteoarthritis rat model

作　　者：郑颖[1] 常新[1] 刘存明[2] 范薇[1] 刘奕[1] 张钧[1] 

机构地区：[1]南京医科大学附属淮安第一医院麻醉科,江苏淮安223300 [2]南京医科大学第一附属医院麻醉科

出　　处：《中华临床医师杂志（电子版）》2013年第18期142-144,共3页Chinese Journal of Clinicians(Electronic Edition)

摘　　要：目的探讨佐剂性诱导骨关节炎大鼠模型血清和脊髓背根神经节中SP表达变化,及其与痛觉评分的相关性和塞来昔布的干预效应。方法将40只SD大鼠随机分为4组:A组(正常组10只)、B组(正常+塞来昔布组10只)、C组(骨关节炎组10只)、D组(骨关节炎+塞来昔布组10只),C和D组予以左膝和尾部皮内分别注射完全弗氏佐剂(CFA)诱导骨关节炎模型,通过酶联免疫吸附测定(ELISA)塞来昔布对于血浆和脊髓背根神经节内SP含量的影响,采用痛觉评分分析塞来昔布对骨关节炎后疼痛行为的影响。结果与对照组比较(A和B组),骨关节炎大鼠模型(C和D组)脊髓和脊髓背根神经节内SP含量明显升高(P<0.01),且SP含量与疼痛疼痛评分呈正相关(r=0.83,P<0.01;r=0.61,P<0.01);并且塞来昔布治疗组(D组)SP含量较C组显著降低(P<0.05)。结论 SP在骨关节炎痛的发生中具有重要作用,塞来昔布抑制SP的合成或释放,从而缓解疼痛。Objective To explore the changes and significance of substance P in dorsal root ganglion and serum, analyze the correlation of substance P and pain score in complete Freund＇s adjuvant （CFA） induced osteoarthritis rat model, and to detect the effect of celecoxib on substance P and pain score. Methods Forty Sprague Dawley rats were randomly divided into four groups with 10 rats in each group：Group A （control）, Group B （celecoxib）, Group C （osteoarthritis）, Group D （osteoarthritis＋celecoxib）. The osteoarthritis rat model in Group C and D were established by using an injection of complete Freund’s adjuvant intradermally at the base of the tail and another intra-articularly （left tibiofemoral）. Contents of substance P in dorsal root ganglion and serum were measured by ELISA（enzyme-linked immunosorbent assay） and the pain were evaluated according to pain score system. Results Contents of substance P in osteoarthritis group （Group C and D） were significantly greater than those in control group （P〈0.01）;Substance P content in dorsal root ganglion and serum in osteoarthritis group was positively correlated with pain scores （r=0.83, P〈0.01;r=0.61, P〈0.01）;The level of substance P content and pain scores were significantly decreased by celecoxib in Group D （P〈0.05）. Conclusion substance P plays an important role in occurrence of pain in osteoarthritis. Celecoxib can inhibit the production of substance P in serum and DRG and ameliorate pain.

关 键 词：骨关节炎 P物质 疼痛 塞来昔布 

分 类 号：R684.3[医药卫生—骨科学]