电针对肾性高血压大鼠脑梗死Rho-A表达的影响  

Effects of Electroacupuncture on Cerebral Rho-A Expressions in Renal Hypertension Rats with Cerebral Infarction

作　　者：谭峰[1] 陈杰[1] 梁艳桂[1] 李雁萍[1] 王学文[1] 蒙迪 程南方[1] 徐丽红[1] 

机构地区：[1]广州中医药大学附属医院佛山中医院,广东佛山528000

出　　处：《新中医》2014年第1期160-164,共5页New Chinese Medicine

基　　金：国家自然科学基金项目(编号:81072947);广东省自然科学基金项目(编号:8152800007000001)

摘　　要：目的:观察电针对易卒中型肾性高血压大鼠(R HR SP)大脑中动脉闭塞(MCAO)后第1、7、14、28天脑梗死灶皮层R ho-A表达的影响。方法:SD大鼠行双肾双夹术复制R HR SP,再用线拴法制作MCAO模型。用随机数字表法分为模型组、电针组、假针刺组、假手术组与高血压组。电针组选取百会、大椎进行电针治疗,每天1次,共28天。假针刺组将针灸针贴于大鼠"百会"和"大椎"穴处皮肤。用尼氏染色检测神经元数目,Western blot检测R ho-A表达。结果:MCAO术后第7、14、28天,电针组神经元数量高于模型组与假针刺组,差异有显著性意义(P<0.05),电针组Rho-A表达低于模型组、假针刺组,差异有显著性意义(P<0.05)。观察第7、14、28天,模型组、电针组、假针刺组R ho-A的表达高于高血压组与假手术组,模型组、电针组、假针刺组神经元数量低于高血压组与假手术组,差异均有显著性意义(P<0.05)。结论:电针对脑梗死中枢神经损伤的保护作用可能与其下调中枢神经生长抑制因子Rho-A表达等机制密切相关。Objective. To observe the effect of electro-acupuncture（EA） on the expression of Rho-A in cerebral infarction cortex of stroke-prone-renal-hypertensive rats（RHRSP） at the 1st 7th, 14th and 21 st day after middle cerebral artery occlusion（MCAO）. Methods： SD rat model of RHRSP was established by bilateral occlusion of bilateral kidney and then by ligatien with the thread. The MCAO models were randomly divided into model group, EA group, sham EA group, sham operation group and hypertension group. EA group was given EA on governor meridian acupoints once a day for 28 days. Sham EA group was given external application of acupuncture needles on Baihui and Dazhui acupoints. Nissl staining was used to examine the number of neurons and Western blot method was used to detect Rho-A expression. Results： At the 7th, 14th and 21st day after MCAO, the neuron count of EA group was higher and Rho-A expression level was lower than those of model group and sham EA group, and the differences were significant（P 〈 0.05）. Model group, EA group and sham EA group had higher Rho-A expression level than hypertension group and sham operation group（P 〈 0.05）. Conclusion： The protective effect of EA on central nerve system injury of cerebral infarction rats may be closely related to the decrease of Rho-A expression.

关 键 词：肾性高血压 电针 脑梗死皮层 Rho—A表达 动物实验 大鼠 

分 类 号：R544.14[医药卫生—心血管疾病]