新型氨基甲酸酯农残生物传感器制备及检测性能分析  

Preparation of a novel carbamate biosensor and analysis its detection performance for analysis of pesticide residues

作　　者：梁东军[1] 郭明[1] 胡润淮[1] 范文翔[1] 

机构地区：[1]浙江农林大学理学院,临安311300

出　　处：《分析试验室》2014年第1期87-91,共5页Chinese Journal of Analysis Laboratory

基　　金：浙江省科技厅项目(2010C33070);浙江省研究生创新基金项目(2012R412052)资助

摘　　要：采用自组装法制备多层乙酰胆碱酯酶／壳聚糖／碳纳米管（AChE／CS／F．CNTs／GCE）生物传感器，利用循环伏安法（CV）、电化学阻抗谱（EIS）和差分脉冲伏安法（DPV）表征传感器并考察其检测氨基甲酸酯农药性能。成功制备了新型氨基甲酸酯ACHE／CS／F．CNTs／GCE生物传感器，新型传感器对氨基甲酸酯农药具有良好的响应。典型氨基甲酸酯类农药2-（1-甲基乙基）苯基甲基氨基甲酸酯在2．00×10^-9～2．00×10^-7g/L内呈现良好的线性关系，检出限达到5．41×10^-9g／L。新型传感器适用于氨基甲酸酯农药残留检测。A new multilayer acetylcholinesterase/ chitosan / carbon nanotubes / glassy carbon electrode （AChE/CS/F-CNTs/GCE） biosensor was prepared by means of self-assembly. It was characterized by cyclic vohammetry （CV）, electrochemical impedance spectrum （EIS） and differential pulse voltammetry （DPV） and the detection performance of carbamate pesticides was investigated. The results showed that the AChE/CS/F- CNTs/GCE biosensor was successful prepared and it had good response. Based on the novel biosensor, the de- tection method was established, it was a quick and easy detection method with wide linear range and high sensi- tivity. The biosensor displayed an excellent electrocatalytical response for detection of isoprocarb and the linear range was 2. 00 ×10^-9-2. 00 ×10^-7g/L, with a correlation coefficient of O. 9830, and the detection limit was 5.41 ×10^-9g/L. The biosensor could be applicable to detection of carbamate pesticide residues.

关 键 词：氨基甲酸酯类农药 乙酰胆碱酯酶 生物传感器 自组装 

分 类 号：O657.1[理学—分析化学]