替吉奥对人肺癌细胞A549及胃癌细胞SGC-7901的体外抑制作用  

Inhibitory effects of S-1 on human lung cancer cell A549 and gastric cancer cell SGC-7901 in vitro

作　　者：胡国英[1] 谢杨芳[1] 周澄宇 

机构地区：[1]江苏省无锡市人民医院药剂科,江苏无锡214023

出　　处：《实用临床医药杂志》2013年第21期125-127,共3页Journal of Clinical Medicine in Practice

基　　金：中国高校医学期刊临床专项资金(11321145)

摘　　要：目的研究替吉奥对人肺癌细胞A549及胃癌细胞SGC-7901的体外抑制作用。方法采用四甲基偶氮唑盐(MTT)测定不同浓度的替吉奥对A549细胞及SGC-7901细胞的抑制作用,并选择5-氟尿嘧啶(5-Fu)作对比,计算半数抑制浓度(IC50)。运用流式细胞仪观察替吉奥对A549及SGC-7901细胞周期和凋亡的影响。结果替吉奥对2种细胞的抑制作用随浓度的增加而增加,呈明显浓度依赖性。替吉奥对A549细胞及SGC-7901细胞的IC50分别为78.33μg/mL和63.40μg/mL,明显低于5-Fu的241.60μg/mL和201.68μg/mL。替吉奥能明显使2种细胞的细胞周期被阻滞于G0/G1及S期,且对A549细胞及SGC-7901细胞的凋亡率分别为(36.64±3.92)%和(38.69±3.02)%,明显高于空白组。结论替吉奥对人肺癌细胞A549及胃癌细胞SGC-7901的体外抑制作用明显,显著优于5-Fu。Objective To study the inhibitory effects of S-1 on human lung cancer cell A549 and gastric cancer cell SGC-7901 in vitro. Methods The inhibitory effects of different concentrations of S-1 on A549 and SGC-7901 cells were detected by methyl thiazolyl tetrazolium （MTT） , and 5-flu- orouracil （5-Fu） was used as a control. Besides, the median inhibitory concentration （IC50） was cal- culated by MTF, and A549 and SGC-7901 cell cycles and apoptosis were observed by flow cytometry. Results The inhibitory effects of S-1 on both cells increased as the concentration went up, and signif- icantly showed concentration-dependant. The IC50 values of S-1 on A549 and SGC-7901 cells were re- spectively lower than those in 5-Fu group （78.33 μg/mL vs 241.60 μg/mL; 63.40 μg/mL vs 201. 68 μg/mL）. It was shown that S-1 can markedly result in the arrest of A549 and SGC-7901 ceils at phases G0/Gl and S, and its apoptosis rates on A549 and SGC-7901 cells were （36.64 ± 3.92） % and （38.69 ± 3.02） %, respectively, evidently higher than in blank group. Conclusion The inhibi- tory effects of S-1 on human lung cancer cell A549 and gastric cancer cell SGC-7901 in vitro are con- spicuous, notably superior to 5-Fu.

关 键 词：替吉奥 A549细胞 SGC-7901细胞 抑制作用 

分 类 号：R979.1[医药卫生—药品]