Effect of Combinations of Sucrose and Cryoprotectants on the Survival of Catfish Embryos （Pangasidae hypophthamus）  

作　　者：Nguyen Thi Hong Nguyen Thi Nhung Nguyen Thi Uoc Nguyen Trung Thanh Nguyen Thi Hiep Nguyen Van Hanh Pham Van Khanh Ha Thi Ngoc Nga Truong Van Thuong Bui Xuan Nguyen 

机构地区：[1]Department of Embryo Biotechnology, Institute of Biotechnology, Vietnam Academy of Science and Technology, 18-Hoang QuocViet, Hanoi 8404, Vietnam [2]Department of Environment and Pathology, Research Institute for Aquaculture No. 2, Cai Be, Tien Giang 8473, Vietnam [3]Research Institute for Aquaculture No. 1, Dinh Bang, Tu Son, Bac Ninh 84241, Vietnam

出　　处：《Journal of Agricultural Science and Technology(B)》2013年第12期887-892,共6页农业科学与技术（B）

摘　　要：Although cryopreservation of embryos has been used in most terrestrial animals, the application of this technique has not been succeeded for aquatic animals. In this study, the authors investigate the effect of different combinations of sucrose （SUC, C12H22OH） and cryoprotectants （CPAs） on the survival of the catfish embryos （Pangasidae hypophthalmus） at low temperatures （4, 0 and -20 ℃） for short-term storage. For this aim, embryos with somites and optic cups were exposed to different combinations of sucrose with methanol （SUC ＋ MeOH）, 1.2-propylene glycol （SUC ＋ PROH） and ethylene glycol （SUC ＋ EG） at four concentrations ratios： （1） 0.5 M SUC ＋ 0.5 M CPA; （2） 1 M SUC ＋ 0.5 M CPA; （3） 0.5 M SUC ＋ 1 M CPA; （4） 1 M SUC ＋ 1 M CPA for 40 min at 4, 0 and -20 ℃. Embryos kept in water at room temperature （RT）, 4, 0 and -20℃ were used as controls. The survival rate was expressed as a percentage of hatched embryos per total embryos treated. The results showed that the hatching rate declined significantly when embryos were stored in water at 0 ℃ and -20℃. For embryos at 0 ℃ storage, the highest survival rate （87.78%） was obtained with 1 M SUC ＋ 1 M MeOH combination while at -20 ℃, only embryos in the combined treatments of 0.5 M SUC ＋ 1 MEG and 0.5 M SUC ＋ 1 M PROH reached the hatching stage （40% and 83.33%, respectively）. In conclusion, the results showed that the catfish embryos are sensitive to sub-zero temperatures and the combined treatment of 0.5 M sucrose and 1 M propylene glycol can be used to protect catfish embryos from damages caused by low temperature （0 ℃ and -20 ℃）.

关 键 词：CATFISH CRYOPROTECTANTS embryos low temperature sucrose. 

分 类 号：S814.8[农业科学—畜牧学] S831.5[农业科学—畜牧兽医]