大鼠自体原位肝移植术后不同时期小肠组织硫氧还蛋白2表达的变化  

Changes in expression of small intestinal thioredoxin 2 during different periods after orthotopic liver auto- transplantation in rats

作　　者：葛缅[1] 池信锦[1] 刘德昭[1] 罗刚健[1] 黄品婕[1] 张瑷兰[1] 黑子清[1] 

机构地区：[1]中山大学附属第三医院麻醉科,广州市510630

出　　处：《中华麻醉学杂志》2013年第11期1315-1317,共3页Chinese Journal of Anesthesiology

基　　金：国家自然科学基金（81170449）;广东省科技计划项目（20118031800078）

摘　　要：目的评价大鼠自体原位肝移植术后不同时期小肠组织硫氧还蛋白2（Trx2）表达的变化。方法健康SPF级sD雄性大鼠40只，8～10周龄，体重210～260g，采用随机数字表法，将其分为2组：假手术组（S组，n=8）和自体原位肝移植术组（OLAT组，n=32）。OLAT组分别在术后4、8、16、24h时取小肠组织，光镜下观察病理学结果，进行Chiu评分，并测定超氧阴离子（O2-）、过氧化氢（H2O2）、谷胱甘肽过氧化物酶（GSH—Px）、还原型谷胱甘肽（GSH）和Trx2水平。结果与s组比较，OLAT组术后4、8、16h时小肠组织Chiu评分和q-活性升高，术后4、8h时小肠组织H2O2含量升高，术后4、8h时小肠组织GSH．Px、GSH水平和Trx2表达降低（P〈0．05）；与术后8h时比较，OLAT组术后4、16、24h时小肠组织Chiu评分降低，术后16、24h时小肠组织H2O2含量降低（P〈0．05）。结论大鼠自体原位肝移植术后经历了小肠Trx2介导的早期抗氧化功能的降低和后期的恢复过程。Objective To evaluate the changes in the expression of small intestinal thioredoxin 2 （Trx2） during different periods after orthotopic liver autotransplantation （OLAT） in rats. Methods Forty Sprague-Dawley rats, aged 8-10 weeks, weighing 210-260 g, were randomly divided into 2 groups using a random number table： sham operation group （group S, n = 8） and OLAT group （ n = 32）. Intestinal tissues were removed at 4, 8, 16 and 24 h after OLAT for microscopic examination and for determination of the levels of superoxide anion （ O2- ）, hydrogen peroxide （H2O2）, glutathione peroxidase （GSH-Px）, reduced glutathione （GSH） and Trx2. Intestinal damage was assessed and scored according to Chiu. Results Compared with S group, the Chiu＇s score and O2- activity at 4, 8 and 16 h after OLAT and H2O2 content at 4 and 8 h after OLAT were significantly increased, and the levels of GSH-Px and GSH and expression of Trx2 at 4 and 8 h after OLAT were decreased in OLAT group （ P 〈 0.05）. Chiu＇ s score at 4, 16 and 24 h after OLAT and H2 02 content at 16 and 24 h after OLAT were signif- icantly lower than those at 8 h in OLAT group （ P 〈 0.05 ）. Conclusion The rats undergo decreased antioxidant capacity in the early phase and recovery in the late phase mediated by small intestinal Trx2 after OLAT.

关 键 词：肝移植 硫氧还蛋白质类 小肠 

分 类 号：R614[医药卫生—麻醉学]