脊髓mTOR在大鼠糖尿病神经痛形成中的作用  

Role of mTOR in spinal cord in development of diabetic neuropathic pain in rats

作　　者：何万友[1] 王汉兵[1] 杨承祥[1] 贺简 杨子文[1] 赵伟成[1] 

机构地区：[1]佛山市第一人民医院麻醉科,528000

出　　处：《中华麻醉学杂志》2013年第11期1359-1361,共3页Chinese Journal of Anesthesiology

基　　金：广东省自然科学基金项目（$2011010003506）

摘　　要：目的评价脊髓mTOR在大鼠糖尿病神经痛形成中的作用。方法健康成年雄性sD大鼠60只，2月龄，体重180—220g，随机取45只大鼠，腹腔注射链脲霉素（STZ）60mg／kg，3d后尾静脉血糖〉16．7mmol／L确定糖尿病造模成功。余下15只大鼠腹腔注射等量的枸橼酸一枸橼酸钠缓冲液作为正常对照组（c组）。于造模前、造模后3、6、9、12、15、18、21d时采用yonFrey纤维丝测定右后足机械性痛阀。糖尿病大鼠造模后21d时机械性痛阈较基础值下降〉50％时纳入糖尿病神经痛组（DP组），而机械性痛阈较基础值下降〈25％时纳入糖尿病非神经痛组（NP组）。于造模后21d，处死大鼠，取脊髓腰膨大组织，采用Westernblot法测定脊髓r胛0R与磷酸化mTOR（p-mTOR）的表达水平。结果与c组比较，DP组和NP组脊髓mTOR表达上调（P〈0．05），DP组脊髓p．mTOR表达上调，NP组脊髓p-mTOR表达差异无统计学意义（P〉0．05）。与NP组比较，DP组P—mlTOR表达上调（P〈0．05），mTOR表达差异无统计学意义（P〉0．05）。结论脊髓mTOR活化参与大鼠糖尿病神经痛的形成。Objective To evaluate the role of mTOR in spinal cord in the development of diabetic neuro- pathic pain in rats. Methods Sixty adult male Sprague-Dawley rats, aged 2 months, weighing 180-220 g, were used in the study. Forty-five rats among them were chosen randomly and diabetes mellitus was induced by intraper- itoneal streptozotocin （STZ） 60 mg/kg and confirmed by blood glucose 〉 16.7 mmol/L on day 3 after STZ injec- tion. The left 15 rats received intraperitoneal injection of the equal volume of citric acid-sodium citrate buffer and served as normal control group （group C） . Paw withdrawal threshold to yon Frey filament stimulation was measured in the right hind paw before STZ injection and on 3, 6, 9, 12, 15, 18, and 21 days after STZ injection. The dia- betic rats with mechanical pain threshold decreasing by more than 50% of the baseline were allocated to diabetic neuropathic pain group （group DP）, and by less than 25 % of the baseline were allocated to diabetic non-neuro- pathic pain group （group NP）. The rats were sacrificed at 21 days after STZ injection, and their lumbar enlarge- ments of the spinal cord were removed for determination of the expression of mTOR and phosphorylated mTOR （p-mTOR） by Western blot. Results The expression of mTOR was significantly up-regulated in DP and NP groups when compared with group C （ P 〈 0.05）, the expression of p-mTOR was up-regulated in DP group, and no sig- nificant change was found in the expression of p-mTOR in group NP （P 〉 0.05）. Compared with group NP, the expression of p-mTOR was significantly up-regulated （ P 〈 0.05）, and no significant change was found in the ex- pression of mTOR in group DP （ P 〉 0.05）. Conclusion Activation of mTOR in the spinal cord is involved in the development of diabetic neuropathic pain in rats.

关 键 词：受体作用蛋白丝氨酸苏氨酸激酶类 糖尿病神经病变 

分 类 号：R587.2[医药卫生—内分泌]