呼吸机相关性肺损伤大鼠肺组织巨噬细胞移动抑制因子mRNA表达的变化  

Change in expression of macrophage migration inhibitory factor mRNA in a rat model of ventilator-induced lung injury

作　　者：戴惠军 潘灵辉[1] 林飞[1] 葛万运[1] 李玮[1] 贺盛[1] 

机构地区：[1]广西医科大学附属肿瘤医院麻醉科,南宁市530021

出　　处：《中华麻醉学杂志》2013年第11期1386-1388,共3页Chinese Journal of Anesthesiology

基　　金：国家自然科学基金（81060008）;2012年广西研究生教育创新计划（YCSZ2012044）

摘　　要：目的探讨呼吸机相关性肺损伤大鼠肺组织巨噬细胞移动抑制因子（MIF）mRNA表达的变化。方法成年雄性sD大鼠30只，体重235～260g，采用随机数字表法，将其分为3组（n=10）：对照组（c组）、小潮气量机械通气组（S组）和大潮气量机械通气组（L组）。C组气管插管后保持自主呼吸；S组和L组气管插管后行机械通气，s组潮气量7ml／kg，L组潮气量40ml／kg，吸呼比1：1，通气频率80次／min，FiO2 100％。自主呼吸或机械通气4h收集支气管肺泡灌洗液，测定总蛋白浓度和WBC计数，采用ELISA法测定MIF、IL-6、IL-1β的浓度；然后处死大鼠，取肺组织，光镜下观察病理学改变，测定湿重／干重比（W／D比），采用RT-PCR测定MIFmRNA表达。结果与C组和s组比较，L组BALF中WBC计数、总蛋白、MIF、IL-6、IL-1β的浓度和肺组织W／D比、MIFmRNA表达升高（P〈0．05），发生病理学损伤；C组和S组间上述各指标比较差异无统计学意义（P〉0．05）。结论MIFinRNA表达上调可能参与了大鼠呼吸机相关性肺损伤的发生。Objective To investigate the changes in the expression of macrophage migration inhibitory factor （MIF） mRNA in a rat model of ventilator-induced lung injury. Methods Thirty adult male Sprague-Dawley rats, weighing 235-260 g, were randomly divided into 3 groups （ n = 10 each） using a random number table： con- trol group （group C）, small tidal volume （VT ） mechanical ventilation group （group S） and large tidal volume me- chanical ventilation group （group L） . The animals were anesthetized with intraperitoneal ketamine 100 mg/kg, mi- dazolam 0.2 mg/kg and atropine 1.0 mg/kg. The rats were tracheostomized and spontaneous breathing was main- tained in group C, while the rats were tracheostomized and mechanically ventilated for 4 h in groups S and L. The tidal volume was 7 ml/kg （group S） or 40 ml/kg （group L）,I： E was 1 ： 1, RR was 80 bpm and FiO2 was 100% . At 4 h of spontaneous breathing or mechanical ventilation, broncho-alveolar lung lavage fluid （BALF） was collect- ed for determination of the total protein concentration; white blood cell （WBC） counts and concentrations of MIF, IL-6 and IL-1β （by ELISA）. Then the rats were sacrificed and the lungs removed for microscopic examination and for determination of wet to dry lung weight ratio （W/D ratio） and expression of MIF mRNA （by RT-PCR）. Results Compared with C and S groups, WBC counts, concentrations of total protein, MIF, IL-6 and IL-1β in BALF, and W/D ratio and expression of MIF mRNA in lung tissues were significantly increased in group L （P 〈 0.05）. There was no significant difference in the indexes mentioned above between group C and group S （ P 〉 0.05 ）. The pathological changes occurred in group L. Conclusion The up-regulation of MIF mRNA expression in lung tissues may be involved in the development of ventilator-induced lung injury in rats.

关 键 词：呼吸 人工 呼吸窘迫综合征 成人 巨噬细胞游走抑制因子 RNA 信使 肺 

分 类 号：R563.8[医药卫生—呼吸系统]