模拟过氧化物酶催化性能的研究  

Studies on Catalytic Characteristic of Mimetic Peroxidase

作　　者：孙淑声[1] 王春海[1] 

机构地区：[1]北京大学化学系,100871

出　　处：《高等学校化学学报》1991年第2期281-283,共3页Chemical Journal of Chinese Universities

基　　金：国家自然科学基金

摘　　要：过氧化物酶(POD)能催化H_2O_2氧化一系列氢供体(DH_2)转化成氧化型供体(D)。氢供体可以是邻联二茴香胺、4-氨基安替比林和苯酚及某些染料隐色体等。这类反应不仅可提供检测H_2O_2的灵敏方法,还可与产生H_2O_2的其它酶反应系统联用,测定葡萄糖、半乳糖、氨基酸、尿酸及胆醇等生物物质。Satio等将锰(Ⅲ)的四-(4-磺酸苯基)卟啉络合物吸附在阴离子交换树脂上,这种被修饰的树脂具有POD的催化活性,从而克服了生物酶价格昂贵和易变性失活的弊端。本文模拟辣根过氧化物酶(HRP)的活性部位,合成了Mn(Ⅲ)与meso-四(3-A mimetic peroxidase, chelate of manganese with tetrakis (3-N-methylpyridyl)-porphyrin was synthesized, and its catalytic characteristic of chromogenic reaction of hydrogen peroxide with 4-aminoantipyrine and N,N-diethylaniline was investigated. A sensitive spectrophotometric method for the determination of trace hydrogen peroxide is developed. The glucose in human serum was determined by the proposed reaction combining with glucose oxidase system with satisfactory results.

关 键 词：过氧化物 酶 催化性 POD 模拟酶 

分 类 号：Q554.6[生物学—生物化学]