铁含量对小胶质细胞释放促炎因子的影响  

The Effect of Cellular Iron Status on Microglia Release Proinflammatory Cytokines

作　　者：庞小翼 

机构地区：[1]四川省绵阳市四0四医院麻醉科,621000

出　　处：《临床合理用药杂志》2013年第36期71-73,共3页Chinese Journal of Clinical Rational Drug Use

摘　　要：目的探索小胶质细胞(MI)内铁含量对MI释放促炎因子的影响。方法使用3,5,5-三甲基乙酰铁(3,5,5-trimethylhexanoyl,TMHF)或去铁胺(deferoxamine,DFO)改变大鼠离体脑MI内铁离子含量,使用ELISA检测上清液中白介素1β(IL-1β)、肿瘤坏死因子α(TNF-α)表达水平及细胞核因子κB(NF-κB)表达数量在MI使用LPS激活前后的变化。结果与对照组相比,LPS激活后MI释放促炎因子增多,并且在铁超载组进一步增多,反之,在铁剥夺组增多程度下降;NF-κB表达在MI被LPS激活后增加,但铁超载MI表达量没有进一步增加。结论激活后MI释放促炎因子量明显受细胞内铁含量影响,但机制可能与NF-κB无关。Objective To investigate cellular iron status influences the function of microglia release proinflammatory cytokines, Methods Microglia were incubated with 3, 5, 5 -trimethylhexanoyl （TMHF） or deferoxamine （DFO） to change the cellular iron status. The levels of TNF - ct, IL - 1 in supernate fluid and the amount of nuclear NF - KB were detected by ELISA before and after induced by LPS. Results Compared with that of control microglia, LPS increases the proinflammatory cytokine release and the amounts of proinflammatory cytokines were enhanced if the microglia were initially iron loaded and dimin- ished if the cells had ~n exposed to an iron chelator. LI~ activation of the microglia significantly increased NF - KB activation, but the addition of iron did not increase the amount of activation; Conclusion The release of proinflammatory cytokines by mi- croglia is affected by their iron status, but the production of cytokines by microglia may have no relation with the activation of NF - xB.

关 键 词：小胶质细胞 铁 炎症 

分 类 号：R614[医药卫生—麻醉学]