人免疫缺陷病毒HBJ株的分离和生物学特性研究  

Isolation of HIV HB-J strain and the observation of its biological characteristics

作　　者：李敬云[1] 鲍作义[1] 王宏霞[1] 罗保君[1] 

机构地区：[1]军事医学科学院微生物学流行病学研究所,北京100071

出　　处：《军事医学科学院院刊》2000年第4期263-264,317,共3页Bulletin of the Academy of Military Medical Sciences

基　　金：北京市科委资助!课题 (95 40 2 0 10 0 )

摘　　要：目的 :分离和鉴定我国流行的人免疫缺陷病毒 (humanimmunodeficiencyvirus ,HIV)毒株 ,研究我国HIV毒株的生物学特性。方法 :用外周血单个核细胞 (peripheralbloodmononuclearcells ,PBMC)共培养的方法分离HIV ,用观察细胞病变、检测P2 4抗原和前病毒DNA的方法监测病毒生长的情况。结果 :在建立共培养的第 12天观察到明显的细胞病变 ,主要表现为大泡样细胞和多核巨细胞。接种第 9天细胞培养上清液的P2 4抗原由阴性转为阳性 ,并且浓度迅速增加 ,接种第 12天浓度即达到 1780pg/ml ,第 14天达到峰值 ,为 2 32 0pg/ml。但该株病毒的PBMC培养上清液转种于MT4细胞后 ,培养上清液P2 4抗原和前病毒DNA始终为阴性且不出现细胞病变。结论 :用PBMC共培养的方法从一名河北籍HIV感染者的PBMC分离出了人免疫缺陷病毒HB J株 ,它是快速生长的、嗜巨噬细胞的、致多核巨细胞形成的毒株 ,而且只感染新鲜的人PBMC而不感染传代人T淋巴细胞MT4 ,可能与使用的辅助受体有关 ,值得深入研究。Objective:To isolate and identify the prevalent HIV strain in China.Methods:The HIV was isolated by co culture with PBMC taken from healthy donor and the virus was monitored by observation on cytopathic effect,P24 antigen and viral DNA. Results:The giant syncytia were observed 12 days after the inoculation. The P24 antigen turned to be detected on day 9 and its concentration in the supernatant of cell culture increased abruptly to 2 320 pg/ml 5 days later. The HB J strain could not infect the MT4 cell as indicated by the negative result of P24 antigen examination of the supernatant and the absence of cytopathological change. Conclusions: A human immunodeficiency virus strain named HB J was isolated by the PBMC co culture method.The difference of co receptor expressed on the surface of PBMC and MT4 may be the reason of the different susceptibility to infection by this HIV strain. [

关 键 词：免疫缺陷病毒 HB-J株 分离 生物学特性 

分 类 号：R373.9[医药卫生—病原生物学] R512.91[医药卫生—基础医学]