基于电聚多巴胺技术一锅法快速构建生物活性界面的研究  

One-pot Preparation of Bioactive Surface by Electropolymerization of Dopamine

作　　者：李紫珺 任科峰[1] 王金磊[1] 计剑[1] 

机构地区：[1]浙江大学高分子科学与工程学系教育部高分子合成与功能构造教育部重点实验室,杭州310027

出　　处：《高分子学报》2014年第1期173-178,共6页Acta Polymerica Sinica

基　　金：国家自然科学基金(基金号51025312,50830106,21174126,51103126);浙江省科技厅优先主题重点社会发展项目(项目号2010c3025-2)资助

摘　　要：通过精确控制电化学参数采用循环伏安法在中性无氧水环境中制备得到膜厚可控的电聚多巴胺膜,并将这种电聚多巴胺技术与生物活性分子的负载相结合,通过一锅法电聚得到含REDV活性短肽的聚多巴胺活性膜,快速便捷地构建了具有促内皮细胞粘附的生物活性界面.椭圆偏振仪、扫描电子显微镜证实了材料界面上形成了均一的聚多巴胺膜;X射线光电子能谱以及荧光分析结果证实了REDV短肽已负载于电聚多巴胺涂层中.内皮细胞体外黏附实验证实REDV短肽保持了良好活性,可有效促进内皮细胞黏附、铺展及粘着斑的形成.这种一锅法快速制备具有生物活性的电聚多巴胺涂层技术有望为复杂的导电生物材料和装置的多功能界面修饰提供新的途径.An electrochemical technique involved facile method of cyclic vohammetric oxidation of dopamine to fabricate thickness controllable PDA films （ePDA） was reported. This process was preceded under a neutral environment and in the absence of oxygen. Most importantly, with precisely controlled electrochemical parameters, this dopamine eleetropolymerization technique can be further combined with bioaetive molecules modification via a one-pot strategy to construct functionalized surfaces. By electropolymerization of dopamine in a one-pot mixture with REDV peptide, an endothelial cells （ECs） specific ligand, ECs adhesion enhanced bioactive surface incorporated with REDV was obtained. Ellipsometry and scanning electron microscopy （SEM） confirmed the homogeneous ePDA film fabrication. X-ray photoelectron spectroscopy （XPS） and fluorescence analysis results demonstrated the successful incorporation of REDV peptide into ePDA films （ePDA@ REDV）. Cells adhesion experiments suggested an excellent activity of incorporated REDV peptide was retained. Furthermore, ECs exhibited improved attachment, spreading and vinculin formation on ePDA@ REDV funetionalized surface. This dopamine eleetropolymerization assisted one-pot strategy for rapid construction of bioactive surfaces provides an innovative approach for multifunctional modification of complicated conductive biomaterials and devices.

关 键 词：电聚合 聚多巴胺 REDV短肽 一锅法 内皮细胞 

分 类 号：O631[理学—高分子化学]