三种蛋白染色方法在SDS-聚丙烯酰胺凝胶电泳中的应用  

Application of three kinds of staining methods for protein in SDS-PAGE

作　　者：陈琳[1] 张亚东[1] 赵艳华[1] 马平[1] 卜凤荣[1] 

机构地区：[1]军事医学科学院野战输血研究所,北京100850

出　　处：《军事医学科学院院刊》2000年第4期292-294,共3页Bulletin of the Academy of Military Medical Sciences

摘　　要：目的 :比较SDS 聚丙烯酰胺凝胶电泳 (SDS -PAGE)中蛋白染色的 3种方法。方法 :将SDS PAGE中的蛋白分别用银染、铜染和考马斯亮蓝染色 ,并对铜染或考马斯亮蓝染色后的蛋白进行相应的复染。结果 :快速银染法灵敏度最高 ,但操作步骤繁琐 ,染色时间约 50min ;考马斯亮蓝染色灵敏度最低 ,需染色和脱色 ,时间约为 1h ;铜染灵敏度介于银染和考马斯亮蓝染色之间 ,但染色时间仅 5min ,不需脱色 ,且可直接用考马斯亮蓝或快速银染法复染。结论 :用SDS PAGE进行蛋白检测时 ,铜染可作为蛋白快速分析的首选方法。Objective: To compare three kinds of staining methods for proteins in SDS PAGE. Methods: The proteins in SDS PAGE were stained with coomassie brilliant blue staining, copper staining and silver staining. Then the proteins were restained after coomassie brilliant blue staining or copper staining. Results:The silver staining was the most sensitive, the sensitivity of the copper staining was the former between that of silver staining and the coomassie brilliant blue staining. It took only five minutes to stain proteins with copper staining. After copper staining, the proteins could be restained by silver staining or coomassie brilliant blue staining. Conclusions: Copper staining could be the first choice for rapid analysis of proteins in SDS PAGE. [

关 键 词：SDS-聚丙烯酰胺 凝胶电泳 蛋白染色方法 

分 类 号：Q503[生物学—生物化学] Q510.3