植物水分生理研究中的压力-容积测定技术几个关键问题  

Key procedures of pressure-volume technique in plant water physiology

作　　者：杨柳[1] 孙慧珍[1] 

机构地区：[1]东北林业大学生态研究中心,哈尔滨150040

出　　处：《生态学杂志》2014年第1期229-234,共6页Chinese Journal of Ecology

基　　金：国家"十二五"科技支撑项目(2011BAD37B01);国家自然科学基金项目(31300507)资助

摘　　要：传统的压力-容积(pressure-volume,PV)技术是通过测定植物组织细胞从吸水饱和状态直至膨压消失的失水全过程中水势与相对含水率,并建立两者之间的关系,从而获得其他方法难以获取的植物水分生理参数(如:质壁分离渗透势、饱和渗透势、质壁分离相对含水量、细胞弹性模量)。这些参数与植物耐旱性密切关联,反映植物组织在干旱期维持功能的能力,因而使该技术在揭示树木因水力衰竭而死亡的生理机制中发挥重要作用。然而,该技术在试验材料预处理、测定、计算方法等关键步骤上尚存在问题。为此,本文针对这些潜在问题,结合中国应用研究现状,对PV技术进行综述,并提出相应的建议。Classic pressure-volume （PV） technique measures water potential and relative water content of plant tissues during the whole process from water saturation to turgor loss and establishes their relationships. Based on these relationships, PV technique has been used to derive such plant water relation parameters as osmotic potential at turgor loss, osmotic potential at full turgor, relative water content at turgor loss, bulk modulus of elasticity, which are difficult to obtain with substitute methods. Because these parameters are strongly associated with plant resistance to drought, the technique will play an important role in understanding physiological mechanisms of droughtinduced tree mortality. However, there are still some inconsistent procedures in its implementing process, such as prerehydration, parameter measurement and calculations. This paper reviews the application of the PV technique by focusing on these inconsistent issues and provides suggestions on appropriate use of the technique in studies of plant water relations.

关 键 词：压力-容积技术 水分参数 水分胁迫 关键问题 

分 类 号：Q945[生物学—植物学]